Three-dimensional segmentation of nuclei and mitotic chromosomes for the study of cell divisions in live Drosophila embryos
Three-dimensional segmentation of nuclei and mitotic chromosomes for the study of cell divisions in live Drosophila embryos
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DOI：
10.1002/cyto.a.21164
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发表时间：
2012-01-01
期刊：
影响因子：
3.7
通讯作者：
Wasser, Martin
中科院分区：
文献类型：
--
作者：
Chinta, Rambabu;Wasser, Martin
果蝇胚胎发生是研究完整多细胞生物体中细胞分裂机制和相关基因的成熟模型。可以使用荧光标记的融合蛋白和延时显微镜在体内监测通过细胞周期阶段的进展。为了测量显微图像中的细胞特性，准确和快速的图像分割方法是一个关键的先决条件。为了量化间期核和有丝分裂染色体的静态和动态特征，我们开发了一种基于多水平集的三维（3D）分割方法。我们在表达组蛋白-2AV-绿色荧光蛋白的活胚胎的3D时间序列图像上测试了我们的方法。我们的方法是强大的低信噪比固有的高速成像，荧光信号在细胞质中，和动态变化的形状和纹理。与手动地面实况分割的比较表明，我们的方法在对象和体素水平上达到了90%以上的准确度，并且在从合胞胚盘到后胚盘有丝分裂域的所有细胞周期阶段和发育阶段都表现一致。(C)2011年国际细胞计数促进学会
Drosophila embryogenesis is an established model to investigate mechanisms and genes related to cell divisions in an intact multicellular organism. Progression through the cell cycle phases can be monitored in vivo using fluorescently labeled fusion proteins and time-lapse microscopy. To measure cellular properties in microscopic images, accurate and fast image segmentation methods are a critical prerequisite. To quantify static and dynamic features of interphase nuclei and mitotic chromosomes, we developed a three-dimensional (3D) segmentation method based on multiple level sets. We tested our method on 3D time-series images of live embryos expressing histone-2Av-green fluorescence protein. Our method is robust to low signal-to-noise ratios inherent to high-speed imaging, fluorescent signals in the cytoplasm, and dynamic changes of shape and texture. Comparisons with manual ground-truth segmentations showed that our method achieves more than 90% accuracy on the object as well as voxel levels and performs consistently throughout all cell cycle phases and developmental stages from syncytial blastoderm to postblastoderm mitotic domains. (C) 2011 International Society for Advancement of Cytometry