Effects of Drynariae rhizoma on the proliferation of human bone cells and the immunomodulatory activity
Effects of Drynariae rhizoma on the proliferation of human bone cells and the immunomodulatory activity
复制标题
DOI：
10.1016/j.phrs.2004.06.005
复制
发表时间：
2005-02-01
影响因子：
9.3
通讯作者：
Kim, CH
中科院分区：
文献类型：
--
作者：
Jeong, JC;Lee, BT;Kim, CH
骨碎补（DR）是一种传统的韩国药物，以其增强肌肉骨骼系统的作用而闻名，经常作为主要成分出现在骨损伤的处方中。然而，目前还不清楚它如何促进骨的重建。本研究首次在体外研究了DR对骨细胞的影响。将人成骨前体细胞（OPC- 1）与不同浓度的DR共同孵育，观察细胞增殖情况。当DR浓度小于或等于120 μ g/ml时，OPC-Ⅰ的增殖增强.而浓度>250 μ g/ml的DR对OPC-Ⅰ的增殖有抑制作用.在大多数处理下，细胞表现出非常苍白的环氧化酶-2（考克斯-2）蛋白表达;在培养过程中，在最高DR浓度120 μ g/ml时显示轻微增强的条带。另一方面，我们研究了DR对细胞免疫和体液免疫的调节作用。给小鼠灌胃不同剂量的DR醇提物和水提物，可剂量依赖性地增强绵羊红细胞（SRBC）和恶唑酮诱导的迟发型超敏反应。它能显著提高SRBC诱导的小鼠循环抗体滴度。但DR对巨噬细胞吞噬功能无明显影响.延长DR给药时间可显著改善总白色血细胞计数，并恢复环磷酰胺诱导的免疫抑制作用。本研究表明，DR具有免疫调节活性。结果表明，DR以剂量依赖性方式直接刺激OPC-1的增殖、碱性磷酸酶活性、蛋白分泌，尤其是I型胶原的合成，并刺激细胞免疫和体液免疫。(C)2004 Elsevier Ltd.保留所有权利。
Drynariae Rhizoma (DR), a traditional Korea medicine, which is known for its effect to strengthen myoskeletal systems, frequently appears as the main ingredient in prescriptions for bone injuries. However, it is unclear how it pharmacologically contributes to the reformation of bone. In this study, the effect of DR on bone cells was investigated in vitro for the first time. The human osteoprecursor cells (OPC- 1) were incubated in the medium with different concentrations of DR and the cell proliferation was studied. When the concentration of DR was less than or equal to 120 mug ml(-1), the proliferation of OPC- I was enhanced. However, the proliferation of OPC- I was inhibited by DR with the concentrations of >250 mug ml(-1). Under most treatments, the cells presented very pale expression for cyclooxygenase-2 (Cox-2) protein; slightly intensified band showed at the highest DR concentration, 120 mug ml(-1) during the course of culture. On the other hand, we investigated the immunomodulatory activity of DR on cellular and Immoral immunity. When different doses of ethanolic and water extracts of DR was administerd to mice, it was dose-dependently potentiated the delayed-type hypersensitivity reaction induced by both sheep red blood cells (SRBC) and oxazolone. It significantly enhanced the production of circulating antibody titre in mice in response to SRBC. But, DR did not any effect on macrophage phagocytosis. Prolonged administration of DR significantly ameliorated the total white blood cell count and also restored the immunosuppressive effects induced by cyclophosphamide. The present investigation reveals that DR possesses immunomodulatory activity. From the results, it was concluded that DR directly stimulated the proliferation, alkaline phosphatase activity, protein secretion and particularly type I collagen synthesis of OPC-1 at dose-dependent manner, and stimulated both the cellular and the humoral immunity. (C) 2004 Elsevier Ltd. All rights reserved.