Radiosensitization of DNA by Cisplatin Adducts Results from an Increase in the Rate Constant for the Reaction with Hydrated Electrons and Formation of Pt
Radiosensitization of DNA by Cisplatin Adducts Results from an Increase in the Rate Constant for the Reaction with Hydrated Electrons and Formation of Pt
复制标题
DOI：
10.1021/acs.jpcb.5b01752
复制
发表时间：
2015-07-30
影响因子：
3.3
通讯作者：
Sanche, L.
中科院分区：
文献类型：
--
作者：
Behmand, B.;Marignier, J. -L.;Sanche, L.
脉冲辐解测量水合..在含有和不含顺铂加合物的不同浓度的寡核苷酸GTG的溶液中的电子表明，顺铂部分的存在加速了水合电子与寡核苷酸之间的反应：反应的速率常数为2.23 × 10 ~（10）mol ~（-1）L·s ~（-1），这表明它是扩散的。控制。此外，我们首次表明，作为水合的选举与GTG-顺铂反应的结果，铂-I中间体的形成。本文提出了一种可能的反应机制，为顺铂联合化疗对癌细胞的放射增敏提供了依据。
Pulse radiolysis measurements of the decay of hydrated.. electrons in solutions containing different concentrations of the oligonucleotide GTG with and without a cisplatin adduct show that the presence of a cisplatin moiety accelerates the reaction between hydrated: electrons and the oligonucleotide: The rate constant of the reaction is foond to be 2.23 x 10(10) mol(-1) L s(-1), which indicates that it is diffusion. controlled. In addition, we show for the first time the formation of a Pt-I intermediate as a result of the reaction of hydrated elections With GTG-cisplatin. A putative reaction mechanism is proposed, Which limy form the basis of the radiosensititation of cancer cells in concomitant chemoratiation therapy with cisplatin.