Structural and functional evolution of three cardiac natriuretic peptides
Structural and functional evolution of three cardiac natriuretic peptides
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DOI：
10.1093/molbev/msi243
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发表时间：
2005-12-01
影响因子：
10.7
通讯作者：
Takei, Y
中科院分区：
文献类型：
--
作者：
Inoue, K;Sakamoto, T;Takei, Y
利钠肽（NPs）是一组在脊椎动物心血管和渗透调节系统中起重要作用的激素。在NP亚型中，心房NP （ANP）、b型NP （BNP）和心室NP （VNP）是仅在心脏中表达的循环激素（心脏NP）。心脏NPs的构成在脊椎动物物种中是可变的。为了了解这种变异的进化和功能意义，我们对生活在不同盐度环境中的9种分类上不同的硬骨鱼的心脏NP cdna进行了系统调查。ANP， BNP和VNP基因在鳗鱼和虹鳟中共存的发现表明，祖先硬骨鱼具有所有三种心脏NPs。由于在ayu和6种Neoteleostei中未检测到VNP cDNA，因此VNP可能是在Osmeroidei分化之前丢失的。在medaka中也检测不到ANP基因。因此，在本研究中，只有BNP基因是普遍存在的。合成medaka BNP优先激活两种medaka gc - a型受体，表明三种心脏NPs共享同一受体。然而，对BNP表达的调控可能是最严格的，因为在硬鱼和四足动物中，atta在3'-非翻译区重复，环上的二元基序是保守的。对虹鳟鱼的连锁分析发现，ANP、BNP和VNP基因位于同一染色体上，表明VNP基因与ANP和BNP基因一样是通过串联重复产生的。如果这种重复发生在四足动物和硬鱼动物分化之前，那么VNP可能存在于四足动物谱系中。
Natriuretic peptides (NPs) are a group of hormones playing important roles in cardiovascular and osmoregulatory systems in vertebrates. Among the NP subtypes, atrial NP (ANP), B-type NP (BNP), and ventricular NP (VNP) are circulating hormones expressed exclusively in the heart (cardiac NPs). The constitution of cardiac NPs is variable among species of vertebrates. In order to understand the evolutionary and functional significance of such variation, we performed a systematic survey of cardiac NP cDNAs in nine taxonomically diverse teleosts inhabiting environments of varying salinity. The discovery of the coexistence of the ANP, BNP, and VNP genes in the eel and rainbow trout suggested that the ancestral teleost had all three cardiac NPs. As the VNP cDNA was undetectable in ayu and six species of Neoteleostei, it is possible that VNP was lost before the divergence of Osmeroidei. The ANP gene was also undetectable in the medaka. Thus, only the BNP gene is universal in species examined in the present study. Synthetic medaka BNP preferentially activated two medaka GC-A-type receptors, suggesting that the three cardiac NPs share the same receptor. However, the regulation of BNP expression may be the most strict because ATTTA repeats in the 3'-untranslated region and the dibasic motif in the ring are conserved among teleosts and tetrapods. Linkage analyses in the rainbow trout located ANP, BNP, and VNP genes on the same chromosome, which suggested the generation of the VNP gene by tandem duplication as observed with ANP and BNP genes. If the duplication occurred before the divergence of tetrapods and teleosts, VNP may exist in the tetrapod lineage.