Transforming growth factor β2 levels in the aqueous humor in different types of glaucoma and the relation to filtering bleb development
Transforming growth factor β2 levels in the aqueous humor in different types of glaucoma and the relation to filtering bleb development
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DOI：
10.1007/s004170000252
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发表时间：
2001-03-01
影响因子：
2.7
通讯作者：
Lütjen-Drecoll, E
中科院分区：
文献类型：
--
作者：
Picht, G;Welge-Luessen, U;Lütjen-Drecoll, E
目的：目的探讨不同类型青光眼[原发性开角型青光眼（POAG）、假性剥脱性青光眼（PSX）、青少年型青光眼（JG）I]房水中转化生长因子β 2（TGF-β 2）和总蛋白水平与小梁切除术后滤过泡形成的关系。研究方法：在手术开始时收集52只青光眼（29只POAG眼，17只PSX眼，6只JG眼）和29只接受白内障手术的对照眼的房水。使用ELISA方法测定TGF-β 2水平（固有活化的和总TGF-β 2）以及房水的总蛋白浓度。所有青光眼术前临床资料（年龄、性别、眼压、既往治疗、手术类型）与TGF-β 2水平进行比较。在其中40只眼中，术后随访（滤过泡发展、干预需求、IOP）与术前确定的TGF-β 2水平相关。结果如下：近一半的POAG患者和大多数JG患者的TGF-β 2水平升高，但在PSX患者中，TGF-β 2水平在正常范围内。TGF-β 2水平与年龄、性别、眼压、既往治疗或手术类型之间无相关性，TGF-β 2水平与房水中蛋白水平之间也无相关性。在13只需要术后干预的眼睛（2型和3型滤过泡）中，8只具有高TGF-β 2水平，5只具有正常TGF-β 2水平。结论：PSX眼与POAG和JG眼的不同之处不仅在于其临床或生物显微镜外观，还在于其房水中的正常TGF-β 2水平。大多数具有良好滤过泡发育的POAG眼具有正常TGF-β 2水平的事实表明，滤过泡的形成与TGF-β 2水平之间可能存在某种关系。另一方面，滤过泡发育不良的眼睛具有低和高TGF-β 2水平的事实表明，其他因素也参与了滤过泡的瘢痕形成。
Purpose: To investigate the transforming growth factor beta2 (TGF-beta2) levels and total protein levels in the aqueous humor of eyes with different types of,glaucoma [primary open-angle glaucoma (POAG), pseudoexfoliation,glaucoma (PSX), juvenile glaucoma (JG)I, and the relation to filtering bleb development after trabeculectomy. Methods: Aqueous humor was collected at the beginning of surgery from 52 eyes with glaucoma (29 POAG eyes, 17 PSX eyes, 6 JG eyes) and from 29 control eyes that underwent cataract operation. TGF-beta2 levels (intrinsically activated and total TGF-beta2) using ELISA methods as well as total protein concentrations of the aqueous humor were determined. All preoperative clinical data of the glaucoma eyes (age, gender, IOP, previous treatment, type of surgery) were compared with the TGF-beta2 levels. In 40 of these eyes, the postoperative follow-up (filtering bleb development, need for intervention, IOP) was correlated to the preoperatively determined TGF-beta2 levels. Results: TGF-beta2 levels were increased in nearly half of the eyes with POAG and in most of the eyes with JG, but in eyes with PSX, TGF-beta2 levels were within the normal range. No correlation between TGF-beta2 levels and age, gender, IOP, previous treatment, or type of surgery, or between TGF-beta2 levels and protein levels in aqueous humor, was found. Correlation between bleb formation and TGF-beta2 levels revealed that all but two of the POAG eyes with good clinical outcome (type 1 bleb) had normal levels of activated TGF-beta2. Of the 13 eyes that needed postoperative intervention (type 2 and type 3 bleb), 8 had high and 5 had normal TGF-beta2 levels. Conclusions: PSX eyes differ from POAG and JG eyes not only by their clinical or biomicroscopic appearance, but also by their normal TGF-beta2 levels in aqueous humor. The fact that most of the POAG eyes with favorable bleb development had normal TGF-beta2 levels indicated that there might be some relationship between bleb formation and TGF-beta2 levels. On the other hand, the fact that eyes with less favorable bleb development had both low and high TGF-beta2 levels indicated that other factors are also involved in the scarring of the filtration bleb.