elt-1, an embryonically expressed Caenorhabditis elegans gene homologous to the GATA transcription factor family.
elt-1, an embryonically expressed Caenorhabditis elegans gene homologous to the GATA transcription factor family.
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elt-1，胚胎表达的秀丽隐杆线虫基因，与 GATA 转录因子家族同源。
DOI：
10.1128/mcb.11.9.4651-4659.1991
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发表时间：
1991
影响因子：
5.3
通讯作者：
Blumenthal,T
中科院分区：
文献类型：
--
作者：
Spieth,J;Shim,YH;Lea,K;Conrad,R;Blumenthal,T
脊椎动物GATA家族转录因子结合的短的、不对称的DNA序列存在于线虫的一些基因调控区：它是激活卵黄蛋白原基因所必需的，也只在trA-2及其基因的TATA盒的5‘端发现。在脊椎动物中，GATA-1是红系特有的，而GATA-2和GATA-3存在于多种组织中。为了确定可能识别该序列元件Inc.的反式作用因子。我们用简并的寡核苷酸克隆了ac。与这种基因同源。我们称之为Geneelt-1(红细胞样转录因子)。它是单拷贝的，指定了一个1.75kb的mRNA，如果不是唯一存在于胚胎中的话，它主要存在于胚胎中。Elt-1编码两个锌指的区域与脊椎动物GATA结合蛋白的DNA结合域非常相似。然而，在DNA结合域之外，氨基酸序列是相当不同的。然而，在elt-1和小鼠GATA-1基因中，内含子位于相同或几乎相同的位置。此外，ELT-1mRNA还与22个碱基的未翻译前导SL1进行了剪接。Elt-1TATA盒上游的DNA在前300bp内含有8个拷贝的GATA识别序列，表明elt-1可能是受自身调节的。我们的结果表明，GATA-1在红系基因表达中的特殊作用是在线虫和导致脊椎动物的品系分离后产生的，因为优雅线虫没有红系血统。
The short, asymmetrical DNA sequence to which the vertebrate GATA family of transcription factors binds is present in someCaenorhabditis elegansgene regulatory regions: it is required for activation of the vitellogenin genes and is also found just 5′ of the TATA boxes oftra-2and themspgenes. In vertebrates GATA-1 is specific to erythroid lineages, whereas GATA-2 and GATA-3 are present in multiple tissues. In an effort to identify thetrans-acting factors that may recognize this sequence element inC. elegans, we used a degenerate oligonucleotide to clone aC. eleganshomolog to this gene. We call this geneelt-1(erythrocytelike transcription factor). It is single copy and specifies a 1.75-kb mRNA that is present predominantly, if not exclusively, in embryos. The region ofelt-1encoding two zinc fingers is remarkably similar to the DNA-binding domain of the vertebrate GATA-binding proteins. However, outside of the DNA-binding domains the amino acid sequences are quite divergent. Nevertheless, introns are located at identical or nearly identical positions inelt-1and the mouse GATA-1 gene. In addition,elt-1mRNA istrans-spliced to the 22-base untranslated leader, SL1. The DNA upstream of theelt-1TATA box contains eight copies of the GATA recognition sequence within the first 300 bp, suggesting thatelt-1may be autogenously regulated. Our results suggest that the specialized role of GATA-1 in erythroid gene expression was derived after separation of the nematodes and the line that led to the vertebrates, since C.eleganslacks an erythroid lineage.