Distribution of Microbial Biomass and Potential for Anaerobic Respiration in Hanford Site 300 Area Subsurface Sediment
Distribution of Microbial Biomass and Potential for Anaerobic Respiration in Hanford Site 300 Area Subsurface Sediment
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DOI：
10.1128/aem.07404-11
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发表时间：
2012-02-01
影响因子：
4.4
通讯作者：
Konopka, Allan
中科院分区：
文献类型：
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作者：
Lin, Xueju;Kennedy, David;Konopka, Allan
为了评估放射性核素污染物的生物地球化学转化潜力，研究人员从华盛顿州东南部汉福德300区的一个52米深钻孔中回收了地下沉积物。对17个沉积物样品进行了微生物分析，这些样品穿越多个地质单元：含氧粗粒Hanford组（9 ~ 17.4 m）、含氧细粒上Ringold组（17.7 ~ 18.1 m）和还原Ringold组（18.3 ~ 52 m）。在不同的样品中，微生物生物量（以磷脂脂肪酸测量）从每克7到974微克不等，在汉福德地层中发现的数量最多。平均而言，17.4 m以下地层的生物量比较浅地层少13倍。编码氧化亚氮还原酶的nosZ基因（通过实时荧光定量PCR测定）相对于总16S rRNA基因在18.3 m和以下的丰度为5 ~ 17%
Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the oxic coarse-grained Hanford formation (9 to 17.4 m), the oxic fine-grained upper Ringold formation (17.7 to 18.1 m), and the reduced Ringold formation (18.3 to 52 m). Microbial biomass (measured as phospholipid fatty acids) ranged from 7 to 974 pmols per g in discrete samples, with the highest numbers found in the Hanford formation. On average, strata below 17.4 m had 13-fold less biomass than those from shallower strata. The nosZ gene that encodes nitrous oxide reductase (measured by quantitative real-time PCR) had an abundance of 5 to 17% relative to that of total 16S rRNA genes below 18.3 m and