Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease
Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease
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DOI：
10.1046/j.1365-2958.2002.03126.x
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发表时间：
2002-09-01
影响因子：
3.6
通讯作者：
Kunst, F
中科院分区：
文献类型：
--
作者：
Glaser, P;Rusniok, C;Kunst, F
无乳链球菌是一种共生菌，在相当大比例的人群肠道中定殖。然而，它也是一种病原体，是新生儿侵袭性感染的主要原因，可导致败血症、脑膜炎和肺炎。我们对血清型III菌株NEM316的基因组进行了测序，该菌株导致了一例致命的败血症病例。其基因组长度为2211485个碱基对，包含2118个蛋白质编码基因。55%的预测基因在化脓性链球菌基因组中有直系同源基因，这代表了这两种链球菌之间的一个保守主链。在无乳链球菌中缺乏化脓性链球菌直系同源基因的基因中，50%聚集在14个岛内。这些岛包含已知的和假定的毒力基因，主要编码表面蛋白以及一些与可移动元件相关的基因。因此，其中一些岛可被视为毒力岛。与其他致病性链球菌相比，无乳链球菌具有独特的特征，即毒力岛可能在毒力获得和遗传多样性方面具有重要作用。
Streptococcus agalactiae is a commensal bacterium colonizing the intestinal tract of a significant proportion of the human population. However, it is also a pathogen which is the leading cause of invasive infections in neonates and causes septicaemia, meningitis and pneumonia. We sequenced the genome of the serogroup III strain NEM316, responsible for a fatal case of septicaemia. The genome is 2 211 485 base pairs long and contains 2118 protein coding genes. Fifty-five per cent of the predicted genes have an ortholog in the Streptococcus pyogenes genome, representing a conserved backbone between these two streptococci. Among the genes in S. agalactiae that lack an ortholog in S. pyogenes, 50% are clustered within 14 islands. These islands contain known and putative virulence genes, mostly encoding surface proteins as well as a number of genes related to mobile elements. Some of these islands could therefore be considered as pathogenicity islands. Compared with other pathogenic streptococci, S. agalactiae shows the unique feature that pathogenicity islands may have an important role in virulence acquisition and in genetic diversity.