Histone concentration regulates the cell cycle and transcription in early development
Histone concentration regulates the cell cycle and transcription in early development
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DOI：
10.1242/dev.177402
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发表时间：
2019-10-01
期刊：
影响因子：
4.6
通讯作者：
Amodeo, Amanda A.
中科院分区：
文献类型：
--
作者：
Chari, Sudarshan;Wilky, Henry;Amodeo, Amanda A.
包括苍蝇、鱼和青蛙在内的许多动物的早期胚胎具有异常快速的细胞周期和延迟的转录启动。这些分裂依赖于母体提供的RNA和蛋白质，包括组蛋白。以前的工作表明，母体提供的组蛋白池的大小可以改变青蛙和鱼的合子基因组激活(ZGA)的时间。在这里，我们研究了母系组蛋白过低和过表达在果蝇胚胎发生中的作用。降低组蛋白浓度可促进合子转录、细胞周期延长、Chk1激活和原肠形成。相反，增加组蛋白浓度会延迟转录，并在原肠形成之前导致额外的核周期。许多合子转录本对组蛋白浓度敏感，组蛋白敏感基因的启动子与特定的染色质特征有关，与组蛋白周转有关。这些问题包括先锋转录因子塞尔达的丰富，以及SIN3A和相关的组蛋白脱乙酰酶的缺乏。我们的发现揭示了果蝇ZGA中组蛋白浓度的关键调节作用。
The early embryos of many animals, including flies, fish and frogs, have unusually rapid cell cycles and delayed onset of transcription. These divisions are dependent on maternally supplied RNAs and proteins including histones. Previous work suggests that the pool size of maternally provided histones can alter the timing of zygotic genome activation (ZGA) in frogs and fish. Here, we examine the effects of under- and overexpression of maternal histones in Drosophila embryogenesis. Decreasing histone concentration advances zygotic transcription, cell cycle elongation, Chk1 activation and gastrulation. Conversely, increasing histone concentration delays transcription and results in an additional nuclear cycle before gastrulation. Numerous zygotic transcripts are sensitive to histone concentration, and the promoters of histone-sensitive genes are associated with specific chromatin features linked to increased histone turnover. These include enrichment of the pioneer transcription factor Zelda, and lack of SIN3A and associated histone deacetylases. Our findings uncover a crucial regulatory role for histone concentrations in ZGA of Drosophila.