Radio-frequency microtools for particle and live cell manipulation
Radio-frequency microtools for particle and live cell manipulation
复制标题
用于颗粒和活细胞操作的射频微型工具
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
A. Heuberger
中科院分区：
文献类型：
--
作者：
G. Fuhr;Torsten Müller;T. Schnelle;R. Hagedorn;Andreas Voigt;Stefan Fiedler;W. Arnold;Ulrich Zimmermann;B. Wagner;A. Heuberger
单粒子可以通过施加高频率的平面结构上制作的超微电极阵列进行操纵。热产生可以减少到甚至可以对未改性的细胞培养基施加强电场的程度。动物细胞在这种连续通电的多电极之间的高场（高达100 kV/m）中正常生长。与激光镊子[1-3]一样，该技术可以捕获场阱中的颗粒和细胞，产生线性运动，并允许细胞培养。它还可以产生pH梯度的微图案，场铸物体，并控制细胞粘附。这些微工具可以结合起来开发细胞分离器，微传感器和可控生物相容性表面。
Single particles can be manipulated by applying high frequencies to ultramicro electrode arrays fabricated on planar structures. Heat production can be reduced to the extent that intense electric fields can be applied even to unmodified cell culture media. Animal cells grow normally in the high field (up to 100 kV/m) between such continuously energized multielectrodes. As with laser tweezers [1-3], this technique can capture particles and cells in field traps, generate linear movement, and permit cell cultivation. It can also produce micropatterns of pH gradients, field-cast objects, and control cell adhesion. These microtools may be combined to develop cell separators, microsensors, and controlled-biocompatibility surfaces.