Variation in time of egg hatch by the honey bee, Apis mellifera (Hymenoptera: Apidae)
Variation in time of egg hatch by the honey bee, Apis mellifera (Hymenoptera: Apidae)
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DOI：
10.1603/0013-8746(2004)097
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发表时间：
2004-01-01
影响因子：
2.3
通讯作者：
Collins, AM
中科院分区：
文献类型：
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作者：
Collins, AM
关于蜜蜂，意大利蜜蜂，卵孵化率和该性状的自然变异是指导蜜蜂胚胎冷冻保存技术发展所必需的。因此，将蜜蜂蜂王在干净的空梳子上关在笼子里4小时，以获得已知年龄的卵组。这些卵是用一个特殊的镊子从梳子上收集的，放在涂有蜂蜡的培养皿上。从产卵后65 h开始观察单个卵，直至孵化（48.6%孵化）。孵化前约2小时可见气管网。然后，观察到直立胚胎的缓慢弯曲和腹部肿胀。在正常孵化中，很少观察到沿胚胎背中线释放液体沿着。相反，经常观察到液体从孵化不良的胚胎的凸起渗出（30.6%）。按照正常的顺序，蛋壳逐渐被消化掉，完成孵化。不同蚁后的卵的年龄差异很大，从66到93 h不等。孵化年龄可能是一个有用的标记，选择更快的发展时间的整体，一个可能的模式，抵抗瓦螨。孵化后1-9小时，幼虫中可见呼吸。将围绕本研究中估计的参数设计通过冷冻保存的胚胎的体外饲养程序。
More detailed information on the age at which a honey bee, Apis mellifera L., egg hatches and the natural variation of this trait was needed to guide development of cryopreservation technology for honey bee embryos. Therefore, honey bee queens were caged on a clean, empty comb for 4 h to obtain groups of eggs of known age. These eggs were collected from the comb using a special forceps and placed on beeswax-coated petri dishes. Individual eggs were observed from 65 h after oviposition until they hatched (48.6% hatched). A tracheal network became visible approximate to2 h before hatching. Then, slow flexing of upright embryos and abdominal peristalsis were seen. Release of a fluid along the dorsal midline of the embryo was observed rarely in normal hatching. In contrast, fluid was frequently observed seeping from bulges on embryos that hatched poorly (30.6%). In a normal sequence, the eggshell was gradually digested away, and complete hatch accomplished. The age at which this occurred was significantly different between eggs from different queens, ranging from 66 to 93 h. Hatching age may be a useful marker for selection of faster development time overall, a possible mode of resistance to the varroa mite. Respiration was visible in the larvae for 1-9 h after hatch. In vitro rearing procedures for embryos preserved by cryopreservation will be designed around the parameters estimated in this study.