Functional analysis of an APSES transcription factor (GlSwi6) involved in fungal growth, fruiting body development and ganoderic-acid biosynthesis in Ganoderma lucidum
Functional analysis of an APSES transcription factor (GlSwi6) involved in fungal growth, fruiting body development and ganoderic-acid biosynthesis in Ganoderma lucidum
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灵芝中参与真菌生长、子实体发育和灵芝酸生物合成的APSES转录因子（GlSwi6）的功能分析
DOI：
10.1016/j.micres.2017.12.015
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发表时间：
2018
影响因子：
6.7
通讯作者：
Zhao Mingwen
中科院分区：
文献类型：
--
作者：
Zhang Guang;Ren Ang;Shi Liang;Zhu Jing;Jiang Ailiang;Shi Dengke;Zhao Mingwen
APSES转录因子被认为是真菌发育和其他生物学过程的关键调节因子。本研究对酿酒酵母Swi6的同源物灵芝GlSwi6的功能进行了研究。利用RNAi技术检测GlSwi6在G. lucidum。沉默GlSwi6导致多种发育缺陷，包括减少真菌生长和增加菌丝分枝，并且GlSwi6沉默菌株没有表现出原基或子实体形成。此外，GlSwi6沉默菌株的H2O2和灵芝酸（GA）水平分别比野生型菌株降低了约50%和25%。此外，H2O2的加入使GlSwi6沉默菌株的GA水平恢复，这意味着GlSwi6可能通过调节细胞内ROS水平来调节GA的生物合成。这些结果表明，GlSwi6参与了G. lucidum。
The APSES transcription factors have been identified as key regulators of fungal development and other biological processes in fungi. In the present study, the function ofGanoderma lucidum GlSwi6, a homolog ofSaccharomyces cerevisiae Swi6, was characterized. RNAi was used to examine the function ofGlSwi6inG. lucidum. SilencingGlSwi6resulted in multiple developmental defects, including reduced fungal growth and increased hyphal branching, and theGlSwi6-silenced strains did not exhibit primordium or fruiting body formation. In addition, the H2O2and ganoderic-acid (GA) levels of theGlSwi6-silenced strains decreased approximately 50% and 25%, respectively, compared with those of the WT strain. Furthermore, the addition of H2O2led to the recovery of the GA levels ofGlSwi6-silenced strains, implying thatGlSwi6might regulate GA biosynthesis by regulating the intracellular ROS levels. Taken together, these results indicate thatGlSwi6is involved in fungal growth, development and GA biosynthesis inG. lucidum.