Extraction and characterization of hemicelluloses from flax shives by different methods
Extraction and characterization of hemicelluloses from flax shives by different methods
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DOI：
10.1016/j.carbpol.2009.06.014
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发表时间：
2010-01-05
影响因子：
11.2
通讯作者：
Mazza, G.
中科院分区：
文献类型：
--
作者：
Buranov, Anvar U.;Mazza, G.
采用加压低极性水（PLPW）、加压乙醇水（PAE）、微波辅助水（MW-Water）或乙醇水（MW-EtOH）从亚麻切片中提取半纤维素，并用乙醇沉淀。用超滤进一步分离溶液中残留的半纤维素。所有样品均采用化学分析、离子慢化分配色谱（IMP）、粒径排除色谱（SEC）和傅里叶变换红外（FT-IR）光谱进行表征。PLPW、PAE、MW-Water和MW-EtOH分别提取了总半纤维素的90%、80%、18%和40%。乙醇沉淀半纤维素的分子量约为11000 ~ 40000 Da，乙醇溶性低分子量半纤维素的分子量约为1700 Da。从PAE提取物中分离出的高分子8半纤维素含有23%的木质素，而从PLPW提取物中分离出的高分子8半纤维素含有5%的木质素。通过超滤从PLPW和PAE提取物中分离出的低分子量半纤维素含有相似量的木质素（接近20%）。然而，与PAE相比，PLPW的低分子量半纤维素收率更高（约为15%）（约为6%）。FT-IR结果显示，木聚糖的最大特异带在1220 cm(-1)，特异带在1175 ~ 1000 cm（-1）之间。(C) 2010年Elsevier Ltd.出版。
Hemicelluloses were extracted from flax shives using pressurized low-polarity water (PLPW), pressurized aqueous ethanol (PAE), microwave-assisted water (MW-Water) or aqueous ethanol (MW-EtOH), and precipitated with ethanol. Hemicelluloses still remaining in solution were further separated using ultrafiltration. All samples were characterized with chemical analysis, ion-moderated partition chromatography (IMP), size exclusion chromatography (SEC), and Fourier transform infrared (FT-IR) spectroscopy. PLPW, PAE, MW-Water and MW-EtOH extracted 90, 80, 18, and 40% of the total hemicelluloses, respectively. The molecular weight of the ethanol-precipitated hemicelluloses ranged from approximately 11,000 to 40,000 Da and the ethanol-soluble low-molecular weight hemicelluloses were about 1700 Da. High-molecular eight hemicellulose isolated from PAE extracts contained similar to 23% lignin, while that from the PLPW extracts contained similar to 5% lignin. Low-molecular weight hemicelluloses separated by ultrafiltration from PLPW and PAE extracts contained similar amounts of lignin (similar to 20%). However, the yield of low-molecular weight hemicelluloses from PLPW was higher (similar to 15%) compared to that from PAE (similar to 6%). The FT-IR results revealed the specific band maximum at 1220 cm(-1) and the bands between 1175 and 1000 cm(-1) which are typical of xylans. (C) 2010 Published by Elsevier Ltd.