Quantitative multiplexed C-reactive protein mass spectrometric immunoassay
Quantitative multiplexed C-reactive protein mass spectrometric immunoassay
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DOI：
10.1021/pr0601133
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发表时间：
2006-07-07
影响因子：
4.4
通讯作者：
Nelson, Randall W.
中科院分区：
文献类型：
--
作者：
Kiernan, Urban A.;Addobbati, Riccardo;Nelson, Randall W.
本文报道了一种用于人血浆c反应蛋白定量分析的高灵敏度质谱免疫分析法的开发和应用。开发了多重亲和检索设备和方法，可同时针对视黄醇结合蛋白、c反应蛋白、血清淀粉样蛋白P成分以及附加的外源性内参标准物（葡萄球菌肠毒素B）进行后续MALDI-TOF质谱分析。该方法允许对视黄醇结合蛋白和血清淀粉样蛋白P组分进行半定量分析，同时对c反应蛋白进行绝对定量测量。定性区分所有三种人类蛋白质及其相关变体的能力也得以维持。高通量分析标准曲线、QC和人血浆样品，其CV < 15%。然后将所得的人血浆样品c反应蛋白定量测量结果与高灵敏度乳胶免疫比浊法测定结果进行比较。
Reported in this work is the development and application of a high sensitivity mass spectrometric immunoassay for the quantitative analysis of C-reactive protein from human plasma. Multiplexed affinity retrieval devices and methodology were developed to simultaneously target retinol binding protein, C-reactive protein, serum amyloid P component, as well as an added exogenous internal reference standard (staphylococcal enterotoxin B) for subsequent MALDI-TOF MS analysis. This approach allows for semiquantitative analysis of both retinol binding protein and serum amyloid P component while performing absolute quantitative measurements of C-reactive protein. The ability to qualitatively differentiate between all three human proteins and their associated variants is also maintained. Standard curve, QC, and human plasma samples were analyzed in a high throughput manner, which performed with a CV < 15%. The resultant human plasma sample C-reactive protein quantitative measurements were then compared to those achieved with a high sensitivity latex immunoturbidimetric assay.