Transcripts and the putative RNA pregenome of duck hepatitis B virus: Implications for reverse transcription
Transcripts and the putative RNA pregenome of duck hepatitis B virus: Implications for reverse transcription
复制标题
鸭乙型肝炎病毒的转录本和假定的 RNA 前基因组：逆转录的意义
DOI：
10.1016/0092-8674(85)90220-x
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发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
H. Schaller
中科院分区：
文献类型：
--
作者：
M. Büscher;W. Reiser;H. Will;H. Schaller
鸭乙型肝炎病毒（DHBV）是一种通过逆转录复制的DNA病毒。我们检查了 DHBV 的转录本，以阐明基因表达和复制的机制。三个主要转录物被表征并分别与核心抗原（DHBcAg）、表面抗原（DHBsAg）和pm-SIDHBs蛋白的基因表达相关。它们是未剪接的并且部分重叠，因为它们起始于不同的启动子位点，但它们终止于共同的聚腺苷酸化信号附近。与 DHBcAg 表达相关的信使 RNA 是唯一超过基因组长度的主要转录物，因此也代表了逆转录（前基因组）的推定模板。它的结构预示着乙型肝炎病毒的复制策略不同于逆转录病毒。
Duck hepatitis B virus (DHBV) is a DNA virus that replicates by reverse transcription. We have examined transcripts of DHBV to elucidate mechanisms of gene expression and replication. Three major transcripts were characterized and related to the expression of the genes for the core antigen (DHBcAg), the surface antigen (DHBsAg), and the pm-SIDHBs protein, respectively. They are unspliced and overlap partially since they start at different promoter sites, but they terminate near a common polyadenylation signal. The messenger RNA related to DHBcAg expression is the only major transcript of more than genome length and therefore also represents the putative template for reverse transcription(pregenome). Its structure predicts a strategy of replication of hepatitis B viruses different from that of retroviruses.