Protective effect of rotavirus VP6-specific IgA monoclonal antibodies that lack neutralizing activity
Protective effect of rotavirus VP6-specific IgA monoclonal antibodies that lack neutralizing activity
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DOI：
10.1126/science.272.5258.104
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发表时间：
1996-04-05
期刊：
影响因子：
56.9
通讯作者：
Greenberg, HB
中科院分区：
文献类型：
--
作者：
Burns, JW;SiadatPajouh, M;Greenberg, HB
轮状病毒是全球幼儿和动物严重胃肠炎和脱水性腹泻的主要原因。使用小鼠模型和“背包瘤”移植来确定针对VP4（外部衣壳病毒蛋白）和VP6（主要内部衣壳病毒蛋白）的抗体的保护作用。只有两个非中和免疫球蛋白A（伊加）抗体VP6能够预防原发性和解决慢性鼠轮状病毒感染。然而，当这些抗体直接呈递到肠道的腔侧时，这些抗体没有活性。这些研究结果支持的假设，在体内细胞内的病毒灭活分泌型伊加转胞吞作用是一种机制的宿主防御轮状病毒感染。
Rotaviruses are the leading cause of severe gastroenteritis and dehydrating diarrhea in young children and animals worldwide. A murine model and ''backpack tumor'' transplantatio n were used to determine the protective effect of anti bod ies against VP4 (an outer capsid viral protein) and VP6 (a major inner capsid viral protein). Only two non-neutralizing immunoglobulin A (IgA) antibodies to VP6 were capable of preventing primary and resolving chronic murine rotavirus infections. These antibodies were not active, however, when presented directly to the luminal side of the intestinal tract. These findings support the hypothesis that in vivo intracellular viral inactivation by secretory IgA during transcytosis is a mechanism of host defense against rotavirus infection.