Quantifying Modes of 3D Cell Migration.
Quantifying Modes of 3D Cell Migration.
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DOI：
10.1016/j.tcb.2015.09.010
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发表时间：
2015-12
影响因子：
19
通讯作者：
Danuser G
中科院分区：
文献类型：
--
作者：
Driscoll MK;Danuser G
虽然人们普遍认为细胞在体内各种不同的环境中迁移，但我们现在才开始使用实验工作流程，产生具有足够时空分辨率的图像，以研究3D环境中控制细胞迁移的分子过程。由于细胞迁移是一个动态过程，通常通过显微镜进行研究，但3D过程的3D电影很难通过目视检查进行解释。在这篇综述中，我们讨论了研究3D细胞迁移模式的多样性所需的技术，重点是研究细胞迁移定量所需的可视化和计算分析工具，其水平与今天在平面基板上爬行的细胞上进行的分析相当。
Although it is widely appreciated that cells migrate in a variety of diverse environments in vivo, we are only now beginning to use experimental workflows that yield images with sufficient spatiotemporal resolution to study the molecular processes governing cell migration in 3D environments. Since cell migration is a dynamic process, it is usually studied via microscopy, but 3D movies of 3D processes are difficult to interpret by visual inspection. In this review, we discuss the technologies required to study the diversity of 3D cell migration modes with a focus on the visualization and computational analysis tools needed to study cell migration quantitatively at a level comparable to the analyses performed today on cells crawling on flat substrates.