Early responses to Salmonella typhimurium infection in mice occur at focal lesions in infected organs
Early responses to Salmonella typhimurium infection in mice occur at focal lesions in infected organs
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DOI：
10.1006/mpat.2000.0405
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发表时间：
2001-01-01
影响因子：
3.8
通讯作者：
Maskell, DJ
中科院分区：
文献类型：
--
作者：
Khan, SA;Strijbos, PJLM;Maskell, DJ
鼠伤寒沙门氏菌在小鼠中引起与人类伤寒相似的侵袭性疾病，在介导宿主对感染的反应中，细胞因子和可能的诱导型一氧化氮合酶（iNOS）具有关键作用。在本文中，我们表明，诱导型一氧化氮合酶的mRNA，蛋白质和酶的活性诱导感染小鼠的脾脏和肝脏内，早在5小时后感染。免疫组织化学和原位杂交表明，iNOS的表达主要发生在巨噬细胞中的局部，离散病灶在感染的器官。未感染的对照小鼠脾和肝中未检测到iNOS活性。使用RT-PCR测量感染器官中编码促炎性细胞因子（TNF α、IL-1 β和IFN γ）的mRNA的存在，所有三种都存在于感染后2小时以后，但在此之前不存在。这些数据表明，有一个非常早期的主机响应S。鼠伤寒杆菌感染，局限于受感染器官内的病灶。(C)北京：科学出版社.
Salmonella typhimurium causes an invasive disease in mice that has similarities to human typhoid, with key roles for cytokines and possibly also inducible nitric oxide synthase (iNOS), in mediating host responses to infection. In this paper we demonstrate that iNOS mRNA, protein and enzyme activity is induced within spleens and livers of infected mice as early as 5 h post-infection. Immunohistochemistry and in situ hybridization indicated that iNOS expression occurs predominantly in macrophages in localized, discrete foci in the infected organs. iNOS activity in spleen and liver was not detectable in uninfected control mice. The presence of mRNA encoding proinflammatory cytokines (TNF alpha, IL-1 beta and IFN gamma) in infected organs was measured using RT-PCR, all three being present from 2 h post-infection onwards, but not before. These data show that there is a very early host response to S. typhimurium infection in mice, limited to foci within the infected organs. (C) 2001 Academic Press.