Transcriptional changes of mouse splenocyte organelle components following acute infection with Toxoplasma gondii
Transcriptional changes of mouse splenocyte organelle components following acute infection with Toxoplasma gondii
复制标题
弓形虫急性感染后小鼠脾细胞细胞器成分的转录变化
DOI：
10.1016/j.exppara.2016.04.019
复制
发表时间：
2016-08-01
影响因子：
2.1
通讯作者：
Zhu, Xing-Quan
中科院分区：
文献类型：
--
作者：
He, Jun-Jun;Ma, Jun;Zhu, Xing-Quan
弓形虫病是一种全球性的人畜共患病。弓形虫是一种能够劫持宿主细胞器进行复制的病原体。尽管许多重要的细胞生命事件都是由细胞器控制的，但人们对T.刚地。在此，我们进行了RNA测序（RNA-seq）和生物信息学分析，以研究全球细胞器组分的变化。结果发现，急性T淋巴细胞白血病小鼠脾细胞器成分的许多转录本发生了改变。RH株弓形虫感染（I型）。大多数线粒体组分的差异表达转录本下调，特别是那些参与生物合成和代谢过程的转录本。此外，基于线粒体的凋亡过程下调。在细胞骨架方面，大部分差异表达的细胞骨架组分的转录本也下调，包括细胞骨架分隔蛋白、细胞骨架组织、中心体和肌球蛋白。对于内溶酶体系统，离子转运蛋白在mRNA水平下调，而细胞溶解组分，如颗粒酶，Rab 27 a和穿孔素1（Prf 1）增加。参与唾液酸化或囊泡介导的运输的高尔基体组分的主要转录物下调，而免疫相关组分上调。对于内质网（ER），与药物代谢和物质转运相关的转录表达下调。此外，T.通过下调CD 76和泛素化相关转录物的表达，可以下调MHC-I复合物对弓形虫抗原的交叉呈递。本研究首次描述了急性T.弓形虫感染，为进一步研究弓形虫与弓形虫之间的相互作用提供了基础。弓形虫和宿主细胞在亚细胞水平。(C)2016 Elsevier Inc. All rights reserved.
Toxoplasmosis is a globally spread zoonosis. The pathogen Toxoplasma gondii can hijack cellular organelles of host for replication. Although a number of important cellular life events are controlled by cell organelles, very little is known of the transcriptional changes of host cellular organelles after infection with T. gondii. Herein, we performed RNA-sequencing (RNA-seq) and bioinformatics analyses to study the global organelle component changes. It was found that many transcripts of the mouse spleen cellular organelle components were altered by acute T. gondii infection with the RH strain (Type I). Most differentially expressed transcripts of mitochondrial components were downregulated, especially those involved in biosynthetic and metabolic processes. Moreover, mitochondria based apoptosis process was downregulated. In terms of cytoskeleton, most differentially expressed transcript of cytoskeleton components were also downregulated, including septin cytoskeleton, cytoskeleton organization, centrosome and myosin. For endolysosomal system, ion transporters were downregulated at mRNA level, whereas the cytolytic components were increased, such as granzymes, Rab27a and perforin1 (Prf1). The main transcripts of Golgi apparatus components involved in sialylation or vesicle-mediated transportation were downregulated, while immune related components were upregulated. For endoplasmic reticulum (ER), posttranslational modification, drug metabolism and material transportation related transcriptswere downregulated. In addition, T. gondii antigen cross-presentation by MHC-I complex could be downregulated by the downregulation of CD76 and ubiquitination related transcripts. The present study, for the first time, described the transcriptional changes of the mouse spleen cellular organelles following acute T. gondii infection, which provides a foundation to study the interaction between T. gondii and host cells at the sub-cellular level. (C) 2016 Elsevier Inc. All rights reserved.