Porphyrin-vancomycin: A highly promising conjugate for the identification and photodynamic inactivation of antibiotic resistant Gram-positive pathogens
Porphyrin-vancomycin: A highly promising conjugate for the identification and photodynamic inactivation of antibiotic resistant Gram-positive pathogens
复制标题
卟啉-万古霉素：一种非常有前途的缀合物，用于鉴定和光动力灭活抗生素耐药性革兰氏阳性病原体
DOI：
10.1016/j.dyepig.2015.04.017
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发表时间：
2015-09-01
影响因子：
4.5
通讯作者：
Yang, Ke-Wu
中科院分区：
文献类型：
--
作者：
Zhai, Le;Yang, Ke-Wu
耐药病原体的出现是一个全球性的公共卫生问题。随着细菌抗生素耐药性的增加，显然需要开发能够有效控制这种情况的替代疗法和材料。本研究成功地探索了一种新型的5，10，15，20-四（对氨基苯基）卟啉-万古霉素偶联物用于鉴定和光灭活耐药革兰氏阳性病原菌。最小抑菌浓度测定和光动力学灭活评价结果表明，该偶联物在白色光下能有效抑制6种供试致病菌的生长。通过表面等离子体共振评价缀合物与革兰氏阳性和革兰氏阴性细菌细胞的相互作用强度，结果表明，在20 mM下，其对金黄色葡萄球菌的活性是其对大肠杆菌的活性的14倍，表明缀合物选择性地聚集在革兰氏阳性细胞中。(C)2015爱思唯尔有限公司版权所有。
The emergence of drug-resistant pathogens is a global public health problem. With the increasing antibiotic resistance of bacteria, there is an obvious need for the development for alternative therapeutics and materials that can effectively control this situation. In this work, a new conjugate, 5,10,15,20-tetrakis (para-aminophenyl) porphyrin-vancomycin was successfully explored for the identification and photoinactivation of antibiotic resistant Gram-positive pathogens. The minimum inhibitory concentration assay and photodynamic inactivation evaluation results revealed that the conjugate can effective inhibit the growth of 6 tested pathogenic strains under white light. The interaction intensity of the conjugate with Gram-positive and Gram-negative bacterial cells was evaluated by surface plasmon resonance, and the results showed that its activity toward Staphylococcus aureus was 14-fold larger than its activity toward Escherichia coli at 20 mM, indicating the conjugate selectively gathers in Gram-positive cells. (C) 2015 Elsevier Ltd. All rights reserved.