Characterization of long-term cultures of hepatitis C virus
Characterization of long-term cultures of hepatitis C virus
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DOI：
10.1128/jvi.70.5.3325-3329.1996
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发表时间：
1996-05-01
影响因子：
5.4
通讯作者：
Shimizu, YK
中科院分区：
文献类型：
--
作者：
Nakajima, N;Hijikata, M;Shimizu, YK
人类 T 细胞系和 B 细胞系 HPBMa10-2 和 Daudi 在感染后 1 年多的时间内产生传染性丙型肝炎病毒 (HCV)。细胞培养物培养的HCV的感染滴度与其基因组滴度相当。蔗糖中的病毒粒子密度约为1.12 g/ml。在接种物中检测到的 13 种变异中，7 种被细胞吸附，并且接种物中少量存在的一种特定 HCV 序列持续存在。
The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus (HCV) for more than 1 year after infection. The infectivity titer of the cell culture-grown HCV and its genome titer were comparable. The virion density in sucrose was around 1.12 g/ml. Among the 13 variants detected in the inoculum, 7 were adsorbed by the cells and one particular HCV sequence which was present in minor quantities in the inoculum persisted.