GABA-immunohistological observations, at the electron-microscopical level, of the neurons of isthmic nuclei in chicken, Gallus domesticus
GABA-immunohistological observations, at the electron-microscopical level, of the neurons of isthmic nuclei in chicken, Gallus domesticus
复制标题
鸡峡部核神经元的电子显微镜水平 GABA 免疫组织学观察
DOI：
10.1007/s004410050995
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
A. Németh
中科院分区：
文献类型：
--
作者：
T. Tömböl;A. Németh
在证明视顶盖内高尔基体浸渍神经元及其终末轴突分支的基础上，用电镜γ-氨基丁酸（GABA）-免疫金染色法对小细胞核和峡部大细胞核的神经元进行了研究。在小细胞核中，没有神经元细胞体或树突显示GABA样免疫反应性的EM制剂染色poststembedded GABA-免疫金。然而，许多GABA样免疫反应和未标记的终端建立与GABA阴性神经元的突触。GABA样免疫反应终末通常见于树突状起源处。在树突状轮廓周围，GABA样免疫反应性和免疫反应阴性终末的孤立突触建立了由胶质细胞过程包围的肾小球样结构。峡部大细胞核的所有巨型和大型神经元均呈GABA样免疫反应。它们的细胞表面完全被GABA样免疫反应阳性和未标记的终末覆盖，这些终末与神经元建立突触。这些神经元被认为将轴突侧支送到小细胞核;它们的轴突进入视顶盖。峡部两核的神经元的形态学特征与中间神经元相似，因为它们有大量的轴体突触，既有不对称的，也有对称的。这些神经元不位于其靶神经元之间，并在一定距离内对视顶盖中的光传输发挥调节作用。
Following a demonstration of Golgi-impregnated neurons and their terminal axon arborization in the optic tectum, the neurons of the nucleus parvocellularis and magnocellularis isthmi were studied by means of postembedded electron-microscopical (EM) γ-aminobutyric acid (GABA)-immunogold staining. In the parvocellular nucleus, none of the neuronal cell bodies or dendrites displayed GABA-like immunoreactivity in EM preparations stained by postembedded GABA-immunogold. However, numerous GABA-like immunoreactive and also unlabeled terminals established synapses with GABA-negative neurons. GABA-like immunoreactive terminals were usually found at the dendritic origin. Around the dendritic profiles, isolated synapses of both GABA-like immunoreactive and immunonegative terminals established glomerulus-like structures enclosed by glial processes. All giant and large neurons of the magnocellular nucleus of the isthmi displayed GABA-like immunoreactivity. Their cell surface was completely covered by GABA-like immunoreactive and unlabeled terminals that established synapses with the neurons. These neurons are thought to send axon collaterals to the parvocellular nucleus; their axons enter the tectum opticum. The morphological characteristics of neurons of both isthmic nuclei are like those of interneurons, because of their numerous axosomatic synapses with both asymmetrical and symmetrical features. These neurons are not located among their target neurons and exert their modulatory effect on optic transmission in the optic tectum at a distance.