Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis: Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses
Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis: Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses
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DOI：
10.1271/bbb.69.2155
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发表时间：
2005-01
期刊：
影响因子：
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通讯作者：
M. Serizawa;K. Kodama;Hiroki Yamamoto;Kazuo Kobayashi;N. Ogasawara;J. Sekiguchi
中科院分区：
文献类型：
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作者：
M. Serizawa;K. Kodama;Hiroki Yamamoto;Kazuo Kobayashi;N. Ogasawara;J. Sekiguchi
YvrGHb是枯草芽孢杆菌中的一种新型双组分系统。yvrG和yvrHb基因被认为分别编码传感激酶和反应调节因子。我们发现YvrGHb系统正调控7个转录单位（wprA、wapA-yxxG、dltABCDE、sunA、sunT-bdbA-yolJ-bdbB、yvrI-yvrHa和sigX-rsiX），负调控lytABC操纵子。wprA、wapA、lytB和lytC编码B的主要细胞表面蛋白。枯草芽孢杆菌此外，与细胞表面稳态功能相关的SigX [细胞质外功能σ因子（ECF σ因子）]也受该系统调节。yvrGHb无效突变株表现出异常的自溶作用，对4种抗生素（氨曲南、头孢吡肟、杆菌肽和磷霉素）的敏感性较高。这些结果表明，YvrGHb系统是重要的，通过转录调节维持细胞表面的整体状态。
YvrGHb is a novel two-component system in Bacillus subtilis. The yvrG and yvrHb genes are considered to encode sensor kinase and response regulator respectively. We found that the YvrGHb system positively regulates the 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sunT-bdbA-yolJ-bdbB, yvrI-yvrHa, and sigX-rsiX), and negatively regulates the lytABC operon. wprA, wapA, lytB, and lytC encode the main cell surface proteins of B. subtilis. Furthermore, SigX [extracytoplasmic function sigma factor (ECF sigma factor)] relating to the cell surface homeostatic functions, was regulated by this system. The yvrGHb null mutant showed the unusual autolysis and higher susceptibility to the four kinds of antibiotics (aztreonam, cefepime, bacitracin, and fosfomycin). These results indicate that the YvrGHb system is important to maintain the overall state of the cell surface through transcriptional regulation.