The cellular mechanism of epithelial rearrangement during morphogenesis of the Caenorhabditis elegans dorsal hypodermis.
The cellular mechanism of epithelial rearrangement during morphogenesis of the Caenorhabditis elegans dorsal hypodermis.
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DOI：
10.1006/dbio.1998.9048
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发表时间：
1998-12
影响因子：
2.7
通讯作者：
Ellen M. Williams-Masson;P. J. Heid;C. Lavin;Jeff Hardin
中科院分区：
文献类型：
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作者：
Ellen M. Williams-Masson;P. J. Heid;C. Lavin;Jeff Hardin
上皮细胞重排的机制是上皮细胞形态发生的核心过程，但仍知之甚少。我们研究了秀丽隐杆线虫胚胎背侧皮下上皮细胞的重排，其中两排上皮细胞在称为背侧嵌套的形态发生过程中重排。插层细胞延伸出基底突起，在它们的粘附连接下挤压在对立的相邻细胞之间。当嵌入细胞向前移动时，这些突出的尖端在前后和背腹的尺寸上变得更宽，有效地“穿过”粘附细胞连接，并迫使剩余的嵌入细胞进入对侧细胞之间。这些细胞运动依赖于完整的细胞结构，因为微管或肌动蛋白丝的药理破坏会阻止细胞重排。这些细胞似乎独立于邻近的皮下细胞插入，因为背侧插入不会被外侧皮下细胞的一半或背侧皮下后半部的祖细胞消融所阻断。这是上皮细胞重排的突出机制首次被表征，我们提出了一个描述上皮细胞如何在上皮单层范围内重排的模型，并讨论了可能指导这些定向细胞运动的机制。
The mechanism by which epithelial cells rearrange is a process that is central to epithelial morphogenesis, yet remains poorly understood. We have investigated epithelial cell rearrangement in the dorsal hypodermis of the Caenorhabditis elegans embryo, in which two rows of epithelial cells rearrange in a morphogenetic process known as dorsal intercalation. The intercalating cells extend basal protrusions which squeeze between their opposing neighbors beneath their adherens junctions. As the intercalating cells move forward, these protruding tips become broader in the anterior-posterior and dorsoventral dimensions, effectively "plowing through" the adherens junctions and forcing an opening for the remainder of the intercalating cell to insert between the contralateral cells. These cell movements are dependent upon intact cytoarchitecture, since the pharmacological disruption of microtubules or actin filaments blocks cell rearrangement. The cells appear to intercalate independently of immediately adjacent neighboring hypodermal cells because dorsal intercalation is not blocked by the ablation of the progenitors for either half of the lateral hypodermal cells or the posterior half of the dorsal hypodermis. This is the first case in which the protrusive mechanism underlying epithelial cell rearrangement has been characterized, and we propose a model describing how epithelial cells rearrange within the confines of an epithelial monolayer, and discuss the mechanisms that may be guiding these directed cell movements.