Effects of individual and combined toxicity of bisphenol A, dibutyl phthalate and cadmium on oxidative stress and genotoxicity in HepG 2 cells
Effects of individual and combined toxicity of bisphenol A, dibutyl phthalate and cadmium on oxidative stress and genotoxicity in HepG 2 cells
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双酚A、邻苯二甲酸二丁酯和镉的单独和联合毒性对HepG 2细胞氧化应激和遗传毒性的影响
DOI：
10.1016/j.fct.2017.03.054
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发表时间：
2017-07-01
影响因子：
4.3
通讯作者：
Zhao, Ling
中科院分区：
文献类型：
--
作者：
Li, Xiaohui;Yin, Pinghe;Zhao, Ling
双酚A、邻苯二甲酸二丁酯和镉可同时存在于环境中。多项研究表明它们具有遗传毒性作用。本研究采用3 × 3全析因设计的单暴露和共暴露处理，研究了不同浓度的单暴露和混合暴露对HepG 2细胞氧化应激和遗传毒性的影响。最高的氧化损伤中观察到镉处理组。与对照组相比，活性氧和丙二醛的最大水平分别接近1.4倍和2.22倍。超氧化物歧化酶活性下降幅度最小，为43%。推测了过度氧化应激导致DNA损伤的机制。然而，BPA处理的细胞表现出最严重的DNA损伤，而不是镉，这可能是因为镉主要破坏DNA修复机制。对于联合效应，由于在混合物毒性研究中阐明了不同的机制，因此不同组合的不同生物学终点中可以发现不同的相互作用。可以肯定的是，与单一暴露相比，联合暴露组的细胞毒性、氧化应激和遗传毒性增强。协同和相加的相互作用被认为是，这意味着更大的威胁生物体暴露于多种雌激素内分泌干扰物。(C)2017爱思唯尔有限公司版权所有
Bisphenol A, dibutyl phthalate and cadmium can be found in environment simultaneously. Several studies suggested that they had genotoxic effect. In this study, mono-exposure and co-exposure treatments, designed by 3 x 3 full factorial, were established to determine the individual toxicity and binary mixtures' combined effects on the oxidative stress and genotoxicity in HepG 2 cells. The highest oxidative damage was observed in the Cd treatments groups. Compared with control groups, the maximum level of reactive oxygen species and malondialdehyde were similar to 1.4 fold and similar to 2.22 fold respectively. And a minimum level of superoxide dismutase activity was found with the decrease of 43%. The mechanism that excessive oxidative stress led to the DNA damage was inferred. However, cells treated with BPA showed the worst DNA damage rather than Cd, which may because Cd mainly damages DNA repairing mechanism. For the joint effect, different interactions can be found in different biological endpoints for different combinations since different mechanisms have been clarified in mixture toxicity studies. It is sure that the co-exposure groups enhanced cytotoxicity, oxidative stress and genotoxicity compared to the mono-exposures. Synergistic and additive interactions were considered, which means greater threat to organisms when exposed to multiple estrogenic endocrine disruptors. (C) 2017 Elsevier Ltd. All rights reserved.