The Polydispersity of αB-Crystallin Is Rationalized by an Interconverting Polyhedral Architecture
The Polydispersity of αB-Crystallin Is Rationalized by an Interconverting Polyhedral Architecture
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DOI：
10.1016/j.str.2011.09.015
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发表时间：
2011-12-07
期刊：
影响因子：
5.7
通讯作者：
Benesch, Justin L. P.
中科院分区：
文献类型：
--
作者：
Baldwin, Andrew J.;Lioe, Hadi;Benesch, Justin L. P.
我们报告了由多分散分子伴侣α b -晶体蛋白填充的最丰富的低聚物的结构模型。亚基连通性通过使用核磁共振光谱和质谱测量获得的约束来确定，从而能够构建各种低聚物模型。根据这些候选结构与离子迁移率光谱数据的对应关系进行过滤，并通过电子显微镜进行交叉验证。随后的最佳匹配结构揭示了α b -结晶蛋白低聚物的多面体结构，并为它们的多分散性和易相互转化提供了基本原理。
We report structural models for the most abundant oligomers populated by the polydisperse molecular chaperone alpha B-crystallin. Subunit connectivity is determined by using restraints obtained from nuclear magnetic resonance spectroscopy and mass spectrometry measurements, enabling the construction of various oligomeric models. These candidate structures are filtered according to their correspondence with ion-mobility spectrometry data and cross-validated by using electron microscopy. The ensuing best-fit structures reveal the polyhedral architecture of alpha B-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion.