Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence
Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence
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DOI：
10.4269/ajtmh.2007.76.950
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Hamburger, Joseph
中科院分区：
文献类型：
--
作者：
Abbasi, Ibrahim;King, Charles H.;Hamburger, Joseph
埃及血吸虫感染了近1.5亿人，主要是在非洲，并通过当地特定种类的Bulinid蜗牛传播。这些螺也可以寄生其他相关的吸虫，因此有效检测和监测感染S。hematobium需要成功区分S.产血吸虫和任何与产血吸虫密切相关的物种。为了能够对S.为了通过简单的聚合酶链反应（PCR）检测血吸虫DNA，我们从大量新发现的血吸虫DNA重复序列中设计并检测了引物对。然而，所有测试的对被发现不适合用于此目的。S.产自S. bovis、牛血吸虫S. mattheei，S. curassoni和S. intercalatum（而不是S. margrebowiei）的扩增最终通过PCR完成，其中使用一个来自新鉴定的重复序列Sh110的引物和第二个来自已知的染色体剪接前导序列的引物。用于评价残留S。控制干预措施后，这种区分工具将使受感染的蜗牛在S。血蜱属与最普遍的其他寄生虫种同域分布。
Schistosoma haematobium infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with S. haematobium requires a successful differentiation between S. haematobium and any closely related schistosome species. To enable differential detection of S. haematobium DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified Schistosoma DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of S. haematobium from S. bovis, S. mattheei, S. curassoni, and S. intercalatum(but not from S. margrebowiei) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual S. haematobium transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where S. haematobium is sympatric with the most prevalent other schistosome species.