Plant origin and ploidy influence gene expression and life cycle characteristics in an invasive weed
Plant origin and ploidy influence gene expression and life cycle characteristics in an invasive weed
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DOI：
10.1186/1471-2229-9-33
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发表时间：
2009-03-23
影响因子：
5.3
通讯作者：
Vivanco, Jorge M.
中科院分区：
文献类型：
--
作者：
Broz, Amanda K.;Manter, Daniel K.;Vivanco, Jorge M.
背景：迄今为止，生态学、进化和生理学的研究提供了一个不完整的画面，为什么一些植物成为入侵；因此，我们利用基因组资源来补充和推进这一领域。为了深入了解半人马花的入侵机制，我们比较了在普通温室环境下生长的三种地细胞型植物，即原生欧亚二倍体、原生欧亚四倍体和引进北美四倍体。我们监测了植物的生产性能特征和生命周期习性，并利用定量PCR技术分析了与组成防御和基因组稳定性相关的基因的表达。结果：植物来源和倍性对植物的生命周期特征和基因表达均有显著影响，突出了在本地和引进植物物种研究中比较合适的分类群的重要性。我们发现，与原生的四倍体相比，引入的C. stoebe种群表现出与构成防御相关的转录本表达减少，这可能是基于敌人释放和快速进化的想法所期望的。几种营养性状的测量结果在所有地细胞型中都是相似的；然而，四倍体的繁殖力明显大于二倍体，部分原因是它们的多倍体性质。对种子生产随时间的模拟预测，引进的四倍体在三种地理细胞型中具有最高的繁殖力。结论：我们的研究结果表明，利用来自其本土和引进范围的种群来表征入侵物种的基因表达，可以深入了解植物入侵的生物学，可以补充传统的植物性能测量方法。此外，这些结果强调了在生态基因组学研究中使用适当的分类单位的重要性。
Background: Ecological, evolutionary and physiological studies have thus far provided an incomplete picture of why some plants become invasive; therefore we used genomic resources to complement and advance this field. In order to gain insight into the invasive mechanism of Centaurea stoebe we compared plants of three geo-cytotypes, native Eurasian diploids, native Eurasian tetraploids and introduced North American tetraploids, grown in a common greenhouse environment. We monitored plant performance characteristics and life cycle habits and characterized the expression of genes related to constitutive defense and genome stability using quantitative PCR.Results: Plant origin and ploidy were found to have a significant effect on both life cycle characteristics and gene expression, highlighting the importance of comparing appropriate taxonomic groups in studies of native and introduced plant species. We found that introduced populations of C. stoebe exhibit reduced expression of transcripts related to constitutive defense relative to their native tetraploid counterparts, as might be expected based on ideas of enemy release and rapid evolution. Measurements of several vegetative traits were similar for all geocytotypes; however, fecundity of tetraploids was significantly greater than diploids, due in part to their polycarpic nature. A simulation of seed production over time predicts that introduced tetraploids have the highest fecundity of the three geo- cytotypes.Conclusion: Our results suggest that characterizing gene expression in an invasive species using populations from both its native and introduced range can provide insight into the biology of plant invasion that can complement traditional measurements of plant performance. In addition, these results highlight the importance of using appropriate taxonomic units in ecological genomics investigations.