Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material
Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material
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DOI：
10.1016/j.foodchem.2008.05.118
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发表时间：
2010-02-15
期刊：
影响因子：
8.8
通讯作者：
Mackay, Ian
中科院分区：
文献类型：
--
作者：
Lee, David;La Mura, Maurizio;Mackay, Ian
混合物样品中转基因RoundUp Ready（TM）大豆的量是使用一种方法测定的，该方法使用信息零进行定量，该方法采用最可能的数字统计来估计分子数量。通过测量这些样品中作为参考标记的凝集素和转基因特异性靶标的数量，我们能够确定它们的转基因含量。来自QUIZ的估计值与使用常规实时Taqman（R）PCR获得的结果相当。使用QUIZ的GMO含量测定结果基于“每个基因组”单位，并且可以使用非常少量的DNA获得估计值。不需要使用重量测量构建的经认证的参考物质进行定量。(C)2008爱思唯尔有限公司保留所有权利。
The amount of genetically modified RoundUp Ready (TM) soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman (R) PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. (C) 2008 Elsevier Ltd. All rights reserved.