Generation of High-Affinity Aptamer-MIP Hybrid Nanoparticles.

Generation of High-Affinity Aptamer-MIP Hybrid Nanoparticles.
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高亲和力适体-MIP 混合纳米颗粒的生成。

DOI:
10.1007/978-1-0716-1629-1_9
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发表时间:
2021
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Sullivan M
Sullivan M
中科院分区:
--
文献类型:
--
作者:
Sullivan M

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适配子和分子印迹聚合物(MIP)是蛋白质仿生学发展的两项主要技术。通过将这两种技术结合起来,可以创建一种新的混合材料类别,它利用了这两种识别材料的有趣特性,同时消除了它们的一些缺点。本章介绍了适体-MIP杂化纳米粒的合成方案。这些材料对其目标模板表现出特殊的亲和力(进入NM范围)和选择性。它们可以针对广泛的目标进行开发,同时在使用中表现出极好的健壮性、溶解性和灵活性。这是一类新型的识别材料,有可能用作药物输送载体,以及用于传感和识别分析。
Aptamers and molecularly imprinted polymers (MIPs) are two leading technologies used for the development of protein biomimetics. By combining the two technologies, a new hybrid class of materials can be created, which utilizes the interesting characteristics of both recognition materials, while negating several of their drawbacks. This chapter describes the protocol for the synthesis of aptamer-MIP hybrid nanoparticles. These materials exhibit exceptional affinity (into the nM range) and selectivity for their target template. They can be developed for a wide range of targets, while exhibiting excellent robustness, solubility, and flexibility in use. These are a new class of recognition materials with the potential for use as drug delivery vectors, as well as use within sensing and recognition assays.
针对 DNA 聚合酶 iota 的高亲和力适体的分离和表征。
DOI: 10.1089/nat.2011.0324
发表时间: 2012
影响因子: 4
作者:
A. V. Lakhin;A. A. Kazakov;A. Makarova;Y. Pavlov;A. Efremova;S. Shram;V. Z. Tarantul;L. Gening
通讯作者: L. Gening
DOI: 10.1039/c4cp01798f
发表时间: 2014-01-01
影响因子: 3.3
作者:
EL-Sharif, Hazim F.;Hawkins, Daniel M.;Reddy, Subrayal M.
通讯作者: Reddy, Subrayal M.