Cryptochromes and Hormone Signal Transduction under Near-Zero Magnetic Fields: New Clues to Magnetic Field Effects in a Rice Planthopper.
Cryptochromes and Hormone Signal Transduction under Near-Zero Magnetic Fields: New Clues to Magnetic Field Effects in a Rice Planthopper.
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近零磁场下的隐花色素和激素信号转导：稻飞虱磁场效应的新线索
DOI：
10.1371/journal.pone.0132966
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen FJ
中科院分区：
文献类型：
--
作者：
Wan GJ;Wang WJ;Xu JJ;Yang QF;Dai MJ;Zhang FJ;Sword GA;Pan WD;Chen FJ
虽然有相当多的报道磁场效应（MFE）对生物体，很少知道到目前为止，关于MFE相关的信号转导途径。在这里，我们建立了一个操纵近零磁场（NZMF），以调查潜在的信号转导通路参与MFE。我们发现，暴露的迁移性白背飞虱，白背飞虱，NZMF的结果在延迟卵和若虫发育，短翅型女性的频率增加，并减少长寿的长翅目女性成年人。为了了解这些表型背后的基因表达变化，我们研究了（i）作为推定的磁传感器的CYP1和CYP2，（ii）保幼激素途径中的JHAMT，FAMeT和JHEH，（iii）蜕皮激素途径中的CYP307A1，以及（iv）生殖相关的卵黄原蛋白（Vg）的基因表达的时间模式。NZMF诱导下，GST1和GST2基因表达的显著改变表明它们的发育阶段特异性模式和磁响应的潜在上游位置。JHAMT、JHEH和CYP307A1的基因表达模式与NZMF触发的若虫发育延迟、短翅型雌虫比例升高和长翅型雌虫寿命缩短一致，表明激素信号转导与表型MFE之间存在可能的联系。通过进行操纵NZMF实验，我们的研究表明，地磁场（GMF）在调节昆虫的发育和生理的重要作用，提供了新的见解MFE磁敏感性相互作用的复杂性，并代表了一个初始的，但关键的一步，在理解隐花色素和激素信号转导的分子基础参与MFE。
Although there are considerable reports of magnetic field effects (MFE) on organisms, very little is known so far about the MFE-related signal transduction pathways. Here we establish a manipulative near-zero magnetic field (NZMF) to investigate the potential signal transduction pathways involved in MFE. We show that exposure of migratory white-backed planthopper, Sogatella furcifera, to the NZMF results in delayed egg and nymphal development, increased frequency of brachypterous females, and reduced longevity of macropterous female adults. To understand the changes in gene expression underlying these phenotypes, we examined the temporal patterns of gene expression of (i) CRY1 and CRY2 as putative magnetosensors, (ii) JHAMT, FAMeT and JHEH in the juvenile hormone pathway, (iii) CYP307A1 in the ecdysone pathway, and (iv) reproduction-related Vitellogenin (Vg). The significantly altered gene expression of CRY1 and CRY2 under the NZMF suggest their developmental stage-specific patterns and potential upstream location in magnetic response. Gene expression patterns of JHAMT, JHEH and CYP307A1 were consistent with the NZMF-triggered delay in nymphal development, higher proportion of brachypterous female adults, and the shortened longevity of macropterous female adults, which show feasible links between hormone signal transduction and phenotypic MFE. By conducting manipulative NZMF experiments, our study suggests an important role of the geomagnetic field (GMF) in modulating development and physiology of insects, provides new insights into the complexity of MFE-magnetosensitivity interactions, and represents an initial but crucial step forward in understanding the molecular basis of cryptochromes and hormone signal transduction involved in MFE.