A new model of visceral pain and referred hyperalgesia in the mouse
A new model of visceral pain and referred hyperalgesia in the mouse
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DOI：
10.1016/s0304-3959(01)00275-5
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发表时间：
2001-06-01
期刊：
影响因子：
7.4
通讯作者：
Cervero, F
中科院分区：
文献类型：
--
作者：
Laird, JMA;Martinez-Caro, L;Cervero, F
缺乏或过度表达与疼痛相关基因的转基因小鼠的产生变得越来越普遍。然而，只有一种内脏疼痛模型，扭体试验，被广泛用于小鼠。在这里，我们描述了一种新的模型，化学刺激结肠，我们已经在小鼠中开发。对任一性别的小鼠静脉注射30 mg/kg伊文思蓝，随后测定血浆外渗。为了进行行为测试，将它们置于凸起的网格上，通过将细套管经肛门插入结肠，给予50穆尔盐水、芥子油（0.25-2.5%）或辣椒素（0.03-0.3%）。内脏疼痛相关的行为（舔腹部，伸展，腹部收缩等）计数20分钟。结肠内给药前，20分钟后，测试的频率撤回反应的应用冯弗雷探针到腹部。死后取出结肠，测量伊文思蓝含量。芥子油和辣椒素管理诱发剂量依赖性内脏疼痛行为，涉及痛觉过敏（显着增加冯弗雷毛的反应）和结肠血浆外渗。0.1%辣椒素和1%芥子油分别诱发最大行为反应。伤害性行为反应被吗啡剂量依赖性地逆转（ED 50 = 1.9 +/-lmg/kg s.c.）我们的结论是，这个模型代表了一个有用的工具，表型突变小鼠和经典药理学，因为信息内脏痛涉及痛觉过敏和结肠炎症都可以从同一只动物。(C)2001 Elsevier Science B. V.由Elsevier Science B. V.出版。保留所有权利。
The generation of transgenic mice that lack or overexpress genes relevant to pain is becoming increasing common. However, only one visceral pain model, the writhing test, is widely used in mice. Here we describe a novel model, chemical stimulation of the colon, which we have developed in mice. Mice of either sex were injected i.v. with 30 mg/kg Evan's Blue for subsequent determination of plasma extravasation. For behavioural testing, they were placed on a raised grid and 50 mul of saline, mustard oil (0.25-2.5%) or capsaicin (0.03-0.3%) was administered by inserting a fine cannula into the colon via the anus. Visceral pain-related behaviors (licking abdomen, stretching, contractions of abdomen etc) were counted for 20 min. Before intracolonic administration, and 20 min after, the frequency of withdrawal responses to the application of von Frey probes to the abdomen was tested. The colon was removed post-mortem and the Evan's Blue content measured. Mustard oil and capsaicin administration evoked dose-dependent visceral pain behaviours, referred hyperalgesia (significant increase in responses to von Frey hairs) and colon plasma extravasation. The peak behavioural responses were evoked by 0.1% capsaicin and by 1% mustard oil respectively. The nociceptive behavioural responses were dose-dependently reversed by morphine (ED50 = 1.9 +/- 1 mg/kg s.c.) We conclude that this model represents a useful tool both for phenotyping mutant mice and for classical pharmacology since information of visceral pain referred hyperalgesia and colon inflammation can all obtained from the same animal. (C) 2001 Elsevier Science B.V. Published by Elsevier Science B.V. All rights reserved.