Analysis of reconstituting T cell receptor repertoires in bone marrow transplant recipients.
Analysis of reconstituting T cell receptor repertoires in bone marrow transplant recipients.
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DOI：
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发表时间：
1995
影响因子：
3.2
通讯作者：
J. Gorski;M. Yassai;C. Keever;N. Flomenberg
中科院分区：
文献类型：
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作者：
J. Gorski;M. Yassai;C. Keever;N. Flomenberg
异基因骨髓移植（BMT）已成为治疗各种造血系统疾病的首选。限制同种异体BMT应用的一个重要因素是免疫功能恢复延迟。基于有效的分析方法对T细胞区室的免疫重建进行定量了解将是有益的。由先前和正在进行的克隆扩增产生的T细胞的不同谱系可以通过其独特的T细胞受体（TCR）来鉴定。因此，TCR复杂性可用作库复杂性的量度。在这里，我们使用聚合酶链反应为基础的方法，可视化的第三互补决定区（CDR 3）的大小异质性研究T细胞重建成人骨髓移植受者。我们发现，剧目的复杂性，由每个V家庭的不同长度的带的数量，反映了测试的个人的一般免疫状态。在患有与T细胞损伤相关的复发性感染的个体中揭示了剧目的收缩和间隙。该方法为分析α/β T细胞库的重建提供了一种新的工具，它也可以应用于B细胞和γ/δ T细胞。
Allogeneic bone marrow transplantation (BMT) has become the treatment of choice for a variety of hematopoietic disorders. An important factor limiting the use of allogeneic BMT is delayed restoration of immune function. A quantitative understanding of immune reconstitution of the T cell compartment based on an efficient method of analysis would be of benefit. Distinct lineages of T cells which have resulted from previous and ongoing clonal expansion can be identified by their unique T cell receptors (TCR). Thus, TCR complexity can be used as a measure of repertoire complexity. Here we use a polymerase chain reaction-based approach which visualizes the size heterogeneity of the third complementarity determining region (CDR3) to study T cell reconstitution in adult bone marrow transplant recipients. We find that repertoire complexity, as determined by the number of bands of different length for each V family, reflects the general immune status of individuals tested. Contractions and gaps in repertoires are revealed in individuals suffering from recurrent infections associated with T cell impairment. This approach provides a new tool in the analysis of reconstitution of alpha/beta T cell repertoires and it can be also be applied to B cells and gamma/delta T cells.