Bicuspid aortic valve hemodynamics does not promote remodeling in porcine aortic wall concavity
Bicuspid aortic valve hemodynamics does not promote remodeling in porcine aortic wall concavity
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DOI：
10.4330/wjc.v8.i1.89
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发表时间：
2016-01-26
影响因子：
1.9
通讯作者：
Sucosky, Philippe
中科院分区：
文献类型：
--
作者：
Atkins, Samantha K.;Moore, Alison N.;Sucosky, Philippe
目的：探讨在无潜在遗传或结构缺陷的情况下，I型左右二叶主动脉瓣（LR-BAV）血流动力学应力在胸升主动脉（AA）瓣口重构中的作用。从正常（非扩张）猪AAs的腹侧切下的组织标本进行48小时的应力环境中使用的剪切应力生物反应器。通过免疫染色和明胶酶谱分析，以基质金属蛋白酶（MMP）的表达和活性为组织重塑的特征。结果：免疫染色半定量结果表明，暴露于TAV和LR-BAV AA WSS的组织组之间MMP-2和MMP-9的表达无显著差异（分别为P = 0.80和P = 0.19）。酶谱光密度法显示MMP-2活性无差异接受TAV AA和LR-BAV AA WSS的组之间的（总活性、活性形式和潜伏形式）（P = 0.08，P = 0.15，P = 0.59）。结论：在I型LR-1的心脏瓣膜中存在的血流动力学应力环境与TAV AA相比，BAV AA不会引起蛋白水解酶表达和活性的任何显著变化。
AIM: To investigate the role of type-I left-right bicuspid aortic valve (LR-BAV) hemodynamic stresses in the remodeling of the thoracic ascending aorta (AA) concavity, in the absence of underlying genetic or structural defects.METHODS: Transient wall shear stress (WSS) profiles in the concavity of tricuspid aortic valve (TAV) and LR-BAV AAs were obtained computationally. Tissue specimens excised from the concavity of normal (nondilated) porcine AAs were subjected for 48 h to those stress environments using a shear stress bioreactor. Tissue remodeling was characterized in terms of matrix metalloproteinase (MMP) expression and activity via immunostaining and gelatin zymography.RESULTS: Immunostaining semi-quantification results indicated no significant difference in MMP-2 and MMP-9 expression between the tissue groups exposed to TAV and LR-BAV AA WSS (P = 0.80 and P = 0.19, respectively). Zymography densitometry revealed no difference in MMP-2 activity (total activity, active form and latent form) between the groups subjected to TAV AA and LR-BAV AA WSS (P = 0.08, P = 0.15 and P = 0.59, respectively).CONCLUSION: The hemodynamic stress environment present in the concavity of type-I LR-BAV AA does not cause any significant change in proteolytic enzyme expression and activity as compared to that present in the TAV AA.