TRPA1 agonists delay gastric emptying in rats through serotonergic pathways
TRPA1 agonists delay gastric emptying in rats through serotonergic pathways
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DOI：
10.1007/s00210-009-0435-7
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发表时间：
2009-10-01
影响因子：
3.6
通讯作者：
Ito, Hiroyuki
中科院分区：
文献类型：
--
作者：
Doihara, Hitoshi;Nozawa, Katsura;Ito, Hiroyuki
我们最近的研究发现，TRPA1在肠嗜铬细胞中高表达，并且在体外用TRPA1激动剂刺激这些细胞可以增加5-羟色胺(5-HT)的分泌。在这里，为了证明TRPA1激动剂在体内的5-羟色胺释放作用，我们检测了TRPA1激动剂对大鼠胃排空的影响。结果表明，TRPA1激动剂呈剂量依赖性地延缓胃排空。此外，在用TRPA1拮抗剂、色氨酸羟化酶抑制剂或5-HT3受体拮抗剂处理的大鼠中，TRPA1激动剂对这一延迟的影响被取消。综上所述，这些结果表明，TRPA1激动剂在体内通过5-羟色胺能途径延缓胃排空。
Our recent study found that TRPA1 is highly expressed in enterochromaffin cells and that stimulation of these cells with TRPA1 agonists enhances 5-hydroxytryptamine (5-HT) secretion in vitro. Here, to demonstrate the 5-HT-releasing effect of TRPA1 agonists in vivo, we examined the effect of TRPA1 agonists on gastric emptying in rats. The results showed that TRPA1 agonists dose-dependently delayed gastric emptying. Further, the effects of TRPA1 agonists on this delay were abolished in rats treated with a TRPA1 antagonist, an inhibitor of tryptophan hydroxylase, or a 5-HT3 receptor antagonist. Taken together, these results indicate that TRPA1 agonists delay in vivo gastric emptying through serotonergic pathways.