Inkjet Printing Methodologies for Drug Screening
Inkjet Printing Methodologies for Drug Screening
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DOI：
10.1021/ac100169w
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发表时间：
2010-04-15
影响因子：
7.4
通讯作者：
Pignataro, Bruno
中科院分区：
文献类型：
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作者：
Arrabito, Giuseppe;Pignataro, Bruno
我们首次展示了一种非接触式、低成本和快速的药物筛选方法，采用喷墨打印进行微阵列格式的分子分配。含有模型底物（d -葡萄糖）/抑制剂（d -葡萄糖）偶联的皮升液滴被精确地分配在由酶靶（葡萄糖氧化酶）共价连接到功能化氧化硅载体的单层上。一种简单的比色检测方法可以证明微阵列的筛选能力，并且可以在单点水平上进行高重复性的分析。光信号作为浓度和时间的函数的测量证实了在固液界面上竞争性酶抑制的发生，该系统在溶液中也发生了类似的行为，同时克服了竞争效应。我们建议将该方法作为药物筛选目的的一般应用，因为它可以扩展到任何类型的酶底物/抑制剂或配体靶生化系统。
We show for the first time a contactless, low-cost, and rapid drug screening methodology by employing inkjet printing for molecular dispensing in a microarray format. Picoliter drops containing a model substrate (D-glucose)/inhibitor (D-glucal) couple were accurately dispensed on a single layer consisting of the enzymatic target (glucose oxidase) covalently linked to a functionalized silicon oxide support. A simple colorimetric detection method allowed one to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Measurements of the optical signal as a function of concentration and of time verified the occurrence at the solid liquid interface of the competitive enzymatic inhibition with a similar behavior occurring for this system in a solution phase along with overcoming competition effects. We propose this methodology as a general application for drug screening purposes, since it may be extended to any kind of enzyme substrate/inhibitor or ligand target biochemical system.