Molecularly imprinted layer-coated monodisperse spherical silica microparticles toward affinity-enrichment of isoflavonoid glycosides from Radix puerariae
Molecularly imprinted layer-coated monodisperse spherical silica microparticles toward affinity-enrichment of isoflavonoid glycosides from Radix puerariae
复制标题
分子印迹层包覆单分散球形二氧化硅微粒亲和富集葛根异黄酮苷
DOI：
10.1039/c2an35049a
复制
发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Zhongping
中科院分区：
文献类型：
--
作者：
Luo, Jing;Zhang, Liying;Zhang, Zhongping
本文报道了葛根素（PR）分子印迹膜的制备及其在硅胶微球上的应用，以实现对PR的选择性识别和对葛根粗提物中主要葛根苷类成分的快速亲和富集。在制备前，利用量子力学方法确定了3种常见的能与PR相互作用的功能单体，并预测了最佳功能单体丙烯酰胺（AA）和模板剂与功能单体的相对摩尔比（PR/AA，1：4）。通过透射电子显微镜（TEM）和重结合实验对所制备的PR分子印迹二氧化硅微粒进行了表征，结果表明，印迹二氧化硅微粒具有良好的形貌和与PR的高结合亲和力，其重结合量是未印迹二氧化硅微粒的近2.1倍。以该印迹微球为固相萃取填料，从葛根粗提液中一步萃取，PR、大豆苷（DD）和染料木苷（GS）的回收率均达到90%以上。此外，PR印迹微粒可以重复使用至少5次而不损失任何提取效率。这些结果表明，PR印迹微球对葛根粗提物中的PR、DD和GS具有高度选择性吸附能力。分子印迹聚合物（MIPs）与固相萃取（SPE）联用技术为复杂结构中药活性成分的富集和分离提供了一种很好的解决方案。
This paper reports the preparation of puerarin (PR) imprinted layer-coated silica microparticles toward selective recognition of PR and fast affinity-enrichment of the main isoflavonoid glycosides from the crude extract of Radix puerariae. Before the preparation, quantum mechanics (QM) method was applied to identify three kinds of common functional monomers capable of interaction with PR and then predicted optimal functional monomer (acrylamide, AA) and the relative molar ratio of template to functional monomer (PR/AA, 1:4). The obtained PR-imprinted silica microparticles were evaluated by transmission electron microscope (TEM) and rebinding experiments, exhibiting good morphology and high binding affinity to PR. Meanwhile, the rebinding amount of the imprinted microparticles to PR was nearly 2.1-folds that of non-imprinted microparticles. When the PR-imprinted microspheres were used as packing materials for solid-phase extraction, the recovery yields of PR, daidzin (DD) and genistin (GS) were simultaneously up to 90% by one-step extraction from the crude extract of Radix puerariae. Additionally, the PR-imprinted microparticles could be re-used for at least 5 times without losing any extraction efficiency. These results indicate that the PR-imprinted microparticles have highly selective adsorption capabilities to PR, DD and GS from the crude extract of Radix puerariae. The method of molecularly imprinted polymers (MIPs) coupled with solid-phase extraction (SPE) provides a good solution of the enrichment and separation of active extracts from complicated traditional Chinese medicine (TCM) with certain structures.