Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells
Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells
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DOI：
10.1091/mbc.7.2.225
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发表时间：
1996-02-01
影响因子：
3.3
通讯作者：
Wang, YL
中科院分区：
文献类型：
--
作者：
Cao, LG;Wang, YL
有丝分裂纺锤体和细胞皮质之间的相互作用被认为在刺激细胞分裂中起关键作用。然而，很少有人了解这种相互作用的性质，特别是在组织培养细胞。我们研究了纺锤体中间区在胞质分裂信号中的作用，方法是用钝针在培养的上皮细胞中建立一个屏障，以阻断可能从该区域发出的信号。当屏障在中期或后期早期形成时，分裂仅发生在皮质面向有丝分裂纺锤体的一侧。分裂侧的微管显示出正常分裂细胞的典型组织。在非分裂的一侧，大多数微管从赤道的一侧进入另一侧，没有任何明显的组织，肌动蛋白丝在赤道区域没有组织。当在分裂后期的第一分钟后形成屏障时，细胞显示出成功的胞质分裂，在屏障两侧具有正常的微管和肌动蛋白丝组织。我们的研究表明，在培养的细胞赤道收缩所需的早期后期纺锤体的中间区的瞬时信号，这种信号可能涉及组织的微管赤道附近。
The interaction between the mitotic spindle and the cellular cortex is thought to play a critical role in stimulating cell cleavage. However, little is understood about the nature of such interactions, particularly in tissue culture cells. We have investigated the role of the spindle midzone in signaling cytokinesis by creating a barrier in cultured epithelial cells with a blunted needle, to block signals that may emanate from this region. When the barrier was created during metaphase or early anaphase, cleavage took place only on the sides of the cortex facing the mitotic spindle. Microtubules on the cleaving side showed organization typical of that in normal dividing cells. On the noncleaving side, most microtubules passed from one side of the equator into the other without any apparent organization, and actin filaments failed to organize in the equatorial region. When the barrier was created after the first minute of anaphase, cells showed successful cytokinesis, with normal organization of microtubules and actin filaments on both sides of the barrier. Our study suggests that transient signals from the midzone of early anaphase spindles are required for equatorial contraction in cultured cells and that such signaling may involve the organization of microtubules near the equator.