Rediscovering Hydrogel-Based Double-Diffusion Systems for Studying Biomineralization.
Rediscovering Hydrogel-Based Double-Diffusion Systems for Studying Biomineralization.
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DOI：
10.1039/c2ce25289a
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发表时间：
2012-01-01
期刊：
影响因子：
3.1
通讯作者：
Estroff LA
中科院分区：
文献类型：
--
作者：
Dorvee JR;Boskey AL;Estroff LA
对于那些寻求体外模拟生物矿化的人来说，水凝胶可以作为细胞外基质（ECM）微环境的优秀模型。实施这种系统的一个主要挑战是从基础工程到实验设计的后勤工作。对于磷酸钙的研究（例如，为了促进羟基磷灰石（hydroxyapatite）的形成，许多研究人员使用基于水凝胶的双扩散系统（DDS）。这些DDS的各种设计似乎与其应用一样独特。在这一亮点中，我们提出了四种不同类型的双扩散系统的调查，并在基本扩散理论的背景下评估它们。基于此分析，我们提出了一个优化系统的设计和评估。这里提出的DDS的评价和构建的技术和框架可以应用于研究人员可能想要实施其特定生物矿化研究的任何DDS。
For those seeking to model biomineralization in vitro, hydrogels can serve as excellent models of the extracellular matrix (ECM) microenvironment. A major challenge posed in implementing such systems is the logistics involved, from fundamental engineering to experimental design. For the study of calcium phosphate (e.g., hydroxyapatite) formation, many researchers use hydrogel-based double-diffusion systems (DDSs). The various designs of these DDSs are seemingly as unique as their applications. In this Highlight, we present a survey of four distinct types of double-diffusion systems and evaluate them in the context of fundamental diffusion theory. Based upon this analysis, we present the design and evaluation of an optimized system. The techniques and framework for the evaluation and construction of a DDS presented here can be applied to any DDS that a researcher may want to implement for their particular studies of biomineralization.