Population Dynamics of Wolbachia in Laodelphax striatellus (Fallen) Under Successive Stress of Antibiotics
Population Dynamics of Wolbachia in Laodelphax striatellus (Fallen) Under Successive Stress of Antibiotics
复制标题
连续抗生素胁迫下灰飞虱（堕落）中沃尔巴克氏体的种群动态
DOI：
10.1007/s00284-019-01762-0
复制
发表时间：
2019-11-01
影响因子：
2.6
通讯作者：
Guo, Huifang
中科院分区：
文献类型：
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作者：
Li, Yongteng;Liu, Xiangdong;Guo, Huifang
Wolbachia是节肢动物中最常见的共生体，抗生素治疗从其宿主中消除共生体是研究其功能所必需的。四环素类抗生素被广泛用于清除昆虫宿主体内的内共生体沃尔巴克氏体。然而，四环素对灰飞虱体内Wolbachia种群数量的影响却知之甚少。在这里，我们调查了沃尔巴克氏体种群密度的动态在女性和男性的L。用实时荧光定量PCR方法检测纹状体细胞。雌、雄鼠体内沃尔巴克氏体密度均高于雄鼠。经2 mg/mL四环素处理1代后，纹状体细胞的DNA含量均急剧下降，再处理1代后，DNA含量继续下降至qPCR和诊断PCR均检测不到的水平，再连续处理3代后，DNA含量维持在0。Wolbachia感染在L.停止四环素处理10代后，这是第一份精确监测L.在连续四环素处理期间和之后的纹状体。这一结果为评价人工操作内共生菌的效率提供了一种有用的方法。
Wolbachia are the most common symbionts in arthropods; antibiotic treatment for eliminating the symbionts from their host is necessary to investigate the functions. Tetracycline antibiotics are widely used to remove endosymbiont Wolbachia from insect hosts. However, very little has been known on the effects of tetracycline on population size of Wolbachia in small brown planthopper (SBPH), Laodelphax striatellus (Fallen), an important insect pest of rice in Asia. Here, we investigated the dynamics of Wolbachia population density in females and males of L. striatellus by real-time fluorescent quantitative PCR method. The Wolbachia density in females and males of L. striatellus all declined sharply after treatment with 2 mg/mL tetracycline for one generation, and continued to decrease to a level which could not be detected by both qPCR and diagnostic PCR after treated for another generation, then maintained at 0 in the following three generations with continuous antibiotic treatment. Wolbachia infection did not recover in L. striatellus after stopping tetracycline treatment for ten generations. This is the first report to precisely monitor the population dynamics of Wolbachia in L. striatellus during successive tetracycline treatment and after that. The results provide a useful method for evaluating the efficiency of artificial operation of endosymbionts.