Microalbuminuria and oxidative stress in essential hypertension
Microalbuminuria and oxidative stress in essential hypertension
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DOI：
10.1046/j.1365-2796.2003.01280.x
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发表时间：
2004-05-01
影响因子：
11.1
通讯作者：
Redón, J
中科院分区：
文献类型：
--
作者：
Giner, V;Tormos, C;Redón, J
Objective.探讨原发性高血压患者微量白蛋白尿与外周血单核细胞氧化应激的关系。共纳入123例未接受抗高血压治疗的高血压患者。使用Spaciros 90207监测仪进行24小时动态血压（BP）监测，并在24小时尿液收集中测量微量白蛋白尿。检测外周血单个核细胞基因组DNA和线粒体DNA中氧化还原型谷胱甘肽（GSH）比值、丙二醛（MDA）含量和受损碱基8-氧代-2 '-脱氧鸟苷（8-oxo-2'-deoxyguanosine，8-ODN）含量。在29例（24%）微量白蛋白尿受试者中，还原型谷胱甘肽的量显著低于正常白蛋白尿受试者，氧化型/还原型谷胱甘肽的比值显著高于正常白蛋白尿受试者。相比之下，同时测量基因组和线粒体DNA氧化的丙二醛和8-氧代-2 '-脱氧鸟苷水平在两组之间没有达到统计学显著性。氧化型/还原型谷胱甘肽比值三分位数最高的受试者显示出最高的尿白蛋白排泄（UAE）（趋势P = 0.04）。多元逐步回归分析显示，当24 h平均血压、年龄、性别、体重指数、血糖和总胆固醇纳入模型时，氧化型/还原型谷胱甘肽比值是UAE的主要显著决定因素，解释9%的方差。氧化应激似乎是UAE的决定因素，即使在高血压受试者中也不依赖于BP水平。
Objective. To assess the relationship between microalbuminuria and oxidative stress in mononuclear peripherals cells in essential hypertension.Methods. A total of 123 hypertensive patients in absence of antihypertensive treatment were included. A 24-h ambulatory blood pressure (BP) monitoring was performed using a Spacelabs 90207 monitor, and microalbuminuria was measured in 24-h urine collections. Oxidized/reduced glutathione ratio and the content of malondialdehide and damaged base 8-oxo-2'-deoxyguanosine in genomic and mitochondrial DNA were measured in peripheral mononuclear cells.Results. In the 29 (24%) microalbuminuric subjects, the amount of reduced glutathione was significantly lower and the ratio oxidized/reduced glutathione was significantly higher than in the normoalbuminuric subjects. In contrast, the simultaneous measurement of the levels of malondialdehide and 8-oxo-2'-deoxyguanosine from both genomic and mitochondrial DNA oxidation did not achieve statistical significance between the two groups. Subjects with the highest oxidized/reduced glutathione ratio tertile showed the highest urinary albumin excretion (UAE) (P = 0.04 for trend). In a stepwise multiple regression analysis, oxidized/reduced glutathione ratio was the main significant determinant of UAE accounting for the 9% of the variance when 24-h mean BP, age, sex, body mass index, glucose and total cholesterol were included in the model.Conclusions. Oxidative stress seems to be a determinant of UAE independent of BP levels even in hypertensive subjects.