Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
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DOI：
10.3390/cells8010056
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发表时间：
2019-01-01
期刊：
影响因子：
6
通讯作者：
de Aro, Andrea Aparecida
中科院分区：
文献类型：
--
作者：
Frauz, Katleen;Teodoro, Luis Felipe R.;de Aro, Andrea Aparecida
组织工程和基于细胞的治疗结合了技术，为细胞存活创造生物相容性材料，从而可以改善肌腱修复。本研究旨在使用一种源自 Crotalus durissus terrificus 毒液的新型纤维蛋白密封剂 (FS)，这是一种仅由动物成分生产的可生物降解的三维支架，与脂肪干细胞 (ASC) 相关，用于治疗肌腱损伤，被认为是常见且严重的骨科问题。 Lewis大鼠的肌腱分布为五组：正常组（N）、横断组（T）、横断组和FS（FS）或ASC（ASC）或FS和ASC（FS + ASC）。体内成像显示，与横断后第 14 天的 ASC 相比，FS + ASC 肌腱中移植的 PKH26 标记的 ASC 定量更高。在横断组的肌腱中观察到少量带有 PKH26 信号的 Iba1 标记巨噬细胞，可能是由于吞噬死亡的 ASC 所致。与 N、T 和 FS 组相比，ASC 上调横切区域的 Tenomodulin 基因表达，并且与 N 组相比，上调 TIMP-2 和 Scleraxis 基因的表达。与 N 组相比，FS 组呈现出更多的胶原纤维组织，其次是 FS + ASC 和 ASC。与 N 组相比，ASC 组的肌腱呈现出更高的羟脯氨酸浓度，并且与 N 组相比，T、FS 和 FS + ASC 的横切肌腱具有更高含量的胶原蛋白 I 和腱调节蛋白。尽管其他生物力学参数没有观察到显着差异，但与ASC组和FS+ASC组相比，T组的最大负荷值更高。总之，直到损伤后第 14 天，FS 保持横切区域移植的 ASC 数量恒定。我们的数据表明，这种 FS 是肌腱修复期间治疗的良好支架，因为它对于肌腱组织恢复而言是最有效的支架，其次是与 ASC 相关的 FS 治疗，最后是第 21 天移植的 ASC。需要对肌腱修复的长期时间点进行进一步研究，以分析 FS 支架发现的更高的组织组织是否会改善肌腱的生物力学。
Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC). The in vivo imaging showed higher quantification of transplanted PKH26-labeled ASC in tendons of FS + ASC compared to ASC on the 14th day after transection. A small number of Iba1 labeled macrophages carrying PKH26 signal, probably due to phagocytosis of dead ASC, were observed in tendons of transected groups. ASC up-regulated the Tenomodulin gene expression in the transection region when compared to N, T and FS groups and the expression of TIMP-2 and Scleraxis genes in relation to the N group. FS group presented a greater organization of collagen fibers, followed by FS + ASC and ASC in comparison to N. Tendons from ASC group presented higher hydroxyproline concentration in relation to N and the transected tendons of T, FS and FS + ASC had a higher amount of collagen I and tenomodulin in comparison to N group. Although no marked differences were observed in the other biomechanical parameters, T group had higher value of maximum load compared to the groups ASC and FS + ASC. In conclusion, the FS kept constant the number of transplanted ASC in the transected region until the 14th day after injury. Our data suggest this FS to be a good scaffold for treatment during tendon repair because it was the most effective one regarding tendon organization recovering, followed by the FS treatment associated with ASC and finally by the transplanted ASC on the 21st day. Further investigations in long-term time points of the tendon repair are needed to analyze if the higher tissue organization found with the FS scaffold will improve the biomechanics of the tendons.