Inhibition of glucagon and insulin secretion by somatostatin in the rat pancreas perfused in situ.
Inhibition of glucagon and insulin secretion by somatostatin in the rat pancreas perfused in situ.
复制标题
原位灌注大鼠胰腺中生长抑素对胰高血糖素和胰岛素分泌的抑制作用。
DOI：
10.1210/endo-96-2-370
复制
发表时间：
1975
期刊：
影响因子：
4.8
通讯作者：
C. J. Goodner
中科院分区：
文献类型：
--
作者：
D. Johnson;J. Ensinck;D. Koerker;J. Palmer;C. J. Goodner
向大鼠胰腺灌注生长激素抑制因子（生长抑素）抑制胰高血糖素和胰岛素分泌到含有5.5 mM葡萄糖的培养基中。精氨酸（20 mM）的15分钟输注大大增加胰高血糖素和胰岛素分泌。当与精氨酸同时灌注时，生长抑素（55 nM）消除了胰高血糖素分泌的增加。精氨酸引起的胰岛素分泌的急性期被生长抑素减弱，随后的分泌减少到对照水平。用生长抑素预处理5min甚至阻断精氨酸引起的急性时相胰岛素分泌。生长抑素不影响基础或葡萄糖刺激的分泌胰岛素从大鼠胰岛分离的胶原酶技术。精氨酸刺激的胰岛素分泌增强生长抑素在离体胰岛。这些结果表明生长抑素对胰腺的直接作用是抑制胰高血糖素和胰岛素的分泌。生长抑素抑制胰岛中胰岛素分泌的失败可能是由于分离过程产生的β细胞的改变。生长抑素对胰岛素分泌的影响也可能是间接介导的。
Perfusion of growth hormone inhibitory factor (somatostatin) into rat pancreas inhibited secretion of glucagon and insulin into medium containing 5.5 mM glucose. A 15-min infusion of arginine (20 mM) greatly increased glucagon and insulin secretion. When perfused simultaneously with arginine, somatostatin (55 nM) abolished the increase in glucagon secretion. The acute phase of insulin secretion in response to arginine was attenuated by somatostatin, and subsequent secretion was decreased to control levels. Pretreatment for 5 min with somatostatin blocked even acute-phase insulin secretion in response to arginine. Somatostatin did not affect basal or glucose-stimulated secretion of insulin from rat pancreatic islets isolated by the collagenase technique. Arginine-stimulated secretion of insulin was enhanced by somatostatin in isolated islets. These results demonstrate a direct effect of somatostatin on the pancreas to inhibit secretion of glucagon and insulin. The failure of somatostatin to inhibit insulin secretion in pancreatic islets may be due to alterations in the beta cells produced by the isolation procedure. It is also possible that the effect of somatostatin on insulin secretion may be mediated indirectly.