Ultra-Wideband Impedance Spectroscopy of a Live Biological Cell
Ultra-Wideband Impedance Spectroscopy of a Live Biological Cell
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DOI：
10.1109/tmtt.2018.2851251
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发表时间：
2018-08-01
影响因子：
4.3
通讯作者：
Hwang, James C. M.
中科院分区：
文献类型：
--
作者：
Ma, Xiao;Du, Xiaotian;Hwang, James C. M.
第一次，活的Jurkat T淋巴细胞人类细胞的阻抗谱被描述为一次扫描，跨越了从9 kHz到9 GHz的60年的频率。超宽带将传统的千兆赫阻抗谱与新近发展的微波介电谱相结合，可以在不受细胞膜阻碍的情况下探测细胞内部。基于测量的散射参数和一个简单的细胞模型，提取了包括膜电阻、膜电容、细胞质电阻和细胞质电容在内的四个非色散元件的等效电路，并发现该等效电路足以解释测量频率上的所谓β驰豫。这些提取的电池参数与文献大体一致，但被认为更准确，给出了相对较小的标准偏差、电池与测量电极密切接触的配置以及测量的超宽带宽。
For the first time, the impedance spectrum of a live Jurkat T-lymphocytes human cell was characterized in a single sweep, spanning six decades of frequency from 9 kHz to 9 GHz. The ultra-wide bandwidth bridged the traditional impedance spectroscopy at kilohertz-megahertz frequencies with the recently developed microwave dielectric spectroscopy, which can probe the cell interior without being hindered by the cell membrane. Based on the measured scattering parameters and a simple cell model, an equivalent circuit of four nondispersive elements, including membrane resistance, membrane capacitance, cytoplasm resistance, and cytoplasm capacitance, was extracted and found sufficient to explain the so-called beta relaxation over the frequencies measured. These extracted cell parameters are in general agreement with the literature but are believed to be more accurate, giving the relatively small standard deviations, the configuration with a cell in intimate contact with the measurement electrodes, and the ultra-wide bandwidth of the measurement.