Neuronal NOS localises to human airway cilia
Neuronal NOS localises to human airway cilia
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DOI：
10.1016/j.niox.2014.11.003
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发表时间：
2015-01-30
影响因子：
3.9
通讯作者：
Lackie, Peter M.
中科院分区：
文献类型：
--
作者：
Jackson, Claire L.;Lucas, Jane S.;Lackie, Peter M.
背景：气道NO合成酶（NOS）同工酶负责快速和局部一氧化氮（NO）的产生，并在气道上皮中表达。由于缺乏证据，我们试图确定神经元NOS （nNOS）在气道上皮中的定位。方法和结果：用甲基丙烯酸乙二醇酯树脂对健康人支气管组织切片和用石蜡对人鼻息肉切片进行免疫组织化学标记，并可重复显示nNOS免疫反应性，特别是在纤毛近端部分；这种免疫反应性被特定的nNOS肽片段阻断。在气液界面（ALI）分化的健康人上皮细胞通过免疫荧光标记证实了所有三种NOS同工酶的存在。只有nNOS的免疫反应性特异于纤毛轴突，并与纤毛标记物β -微管蛋白在纤毛轴突近端共定位。结论：我们报道了nNOS在气道上皮细胞纤毛近端部分的新定位，并得出结论，其独立和局部调节NO水平对正常纤毛功能至关重要。(C) 2014爱思唯尔公司版权所有。
Background: Airway NO synthase (NOS) isoenzymes are responsible for rapid and localised nitric oxide (NO) production and are expressed in airway epithelium. We sought to determine the localisation of neuronal NOS (nNOS) in airway epithelium due to the paucity of evidence.Methods and results: Sections of healthy human bronchial tissue in glycol methacrylate resin and human nasal polyps in paraffin wax were immunohistochemically labelled and reproducibly demonstrated nNOS immunoreactivity, particularly at the proximal portion of cilia; this immunoreactivity was blocked by a specific nNOS peptide fragment. Healthy human epithelial cells differentiated at an air-liquid interface (ALI) confirmed the presence of all three NOS isoenzymes by immunofiuorescence labelling. Only nNOS immunoreactivity was specific to the ciliary axonemeand co-localised with the cilia marker beta-tubulin in the proximal part of the ciliary axoneme.Conclusions: We report a novel localisation of nNOS at the proximal portion of cilia in airway epitheliurn and conclude that its independent and local regulation of NO levels is crucial for normal cilia function. (C) 2014 Elsevier Inc. All rights reserved.