LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast
LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast
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DOI：
10.1016/j.bbrc.2003.10.110
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发表时间：
2003-11-28
影响因子：
3.1
通讯作者：
Park, HM
中科院分区：
文献类型：
--
作者：
Park, YD;Kang, WH;Park, HM
以前，我们报告说，LAMMER激酶同源物，Lkh 1，是一个负调节器的丝状生长和无性絮凝的裂殖酵母，裂殖酵母。在这里，我们报告说，lkh 1（+）无效突变体是敏感的氧化应激，因为在抗氧化酶，如过氧化氢酶（cttl（+））和铜，锌-超氧化物歧化酶（sod 1（+））的基因表达减少。此外，与野生型细胞相比，Ikh 1（+）无效突变体在氧化应激条件下显示出细胞内过氧化物水平增加。有趣的是，在氧化应激下，lkh 1（+）无效突变体中转录因子Atf 1的基因表达降低，而转录因子Pap 1的表达则没有。我们报告了新的发现，Lkh 1参与了氧化应激反应的裂变酵母，S。pombe，并通过转录因子Atf 1调节抗氧化酶的表达。(C)2003年爱思唯尔公司All rights reserved.
Previously, we reported that the LAMMER kinase homolog, Lkh1, is a negative regulator of filamentous growth and asexual flocculation in the fission yeast, Schizosaccharomyces pombe. Here, we report that the lkh1(+) null mutant is sensitive to oxidative stress because of a reduction in the expression of genes for antioxidant enzymes such as catalase (cttl(+)) and Cu,Zn-superoxide dismutase (sod1(+)). Furthermore, the Ikh1(+) null mutant shows increased levels of intracellular peroxides under conditions of oxidative stress compared with wild-type cells. Interestingly, expression of the gene for the transcription factor Atf1 is reduced in the lkh1(+) null mutant under oxidative stress, whereas expression of the transcription factor Pap1 is not. We report the novel finding that Lkh1 is involved in the oxidative-stress response of the fission yeast, S. pombe, and regulates the expression of antioxidant enzymes via the transcription factor Atf1. (C) 2003 Elsevier Inc. All rights reserved.