ACTIVATION OF GLOBIN GENES DURING CHICKEN DEVELOPMENT
ACTIVATION OF GLOBIN GENES DURING CHICKEN DEVELOPMENT
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DOI：
10.1016/0092-8674(81)90329-9
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
GROUDINE, M;WEINTRAUB, H
孵化35h时，Hb首先出现在发育中的鸡胚层中。分离了胚胎20-23h的Hb形成区，结果表明该区域的大部分细胞是红细胞前体。这些前体中的珠蛋白基因的结构和活性通过三种方法进行分析：通过径流核转录来测量珠蛋白基因上存在的启动的RNA聚合酶的数量；通过测量这些基因与甲基敏感的限制性内切酶的甲基化程度；通过监测DNAase I的敏感性和珠蛋白染色质中DNAase I过敏性位点的产生。所有这些检测都显示珠蛋白基因在前体细胞中不活跃。对染色体结构从不活跃到活跃的转变机制的其他实验表明，DNA复制而不是胞质分裂与转变过程密切相关。
Hb first appears in the developing chicken blastoderm at 35 h incubation. The presumptive Hb-forming area of the embryo at 20-23 h was isolated and shown that most of the cells in this region are red cell precursors. The structure and activity of the globin genes in these precursors were assayed in 3 ways: by runoff nuclear transcription to measure the number of initiated RNA polymerases present on the globin genes; by measuring the degree of methylation of these genes with methyl-sensitive restriction enzymes and by monitoring the DNAase I sensitivity and the generation of DNAase I-hypersensitivite sites in the globin chromatin. All these assays show the globin genes inactive in precursor cells. Additional experiments focusing on the mechanism of the transition from an inactive to an active chromosome structure indicate that DNA replication but not cytokinesis is intimately coupled to the transition process.