Viral superantigen drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.
Viral superantigen drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.
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DOI：
10.1084/jem.185.3.551
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发表时间：
1997-02-03
期刊：
影响因子：
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通讯作者：
MacLennan IC
中科院分区：
文献类型：
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作者：
Luther SA;Gulbranson-Judge A;Acha-Orbea H;MacLennan IC
小鼠乳腺肿瘤病毒（MMTV[SW]）编码由感染的B细胞表达的超抗原。它引起对病毒包膜蛋白特异性的抗体应答，表明抗原特异性B细胞的选择性活化。使用流式细胞术和免疫组织学比较引流淋巴结中对MMTV（SW）的反应与对半抗原鸡丙种球蛋白（NP-CGG）的反应。在两种应答中均发生T细胞引发，其中T细胞与T区中的交错树突状细胞相关联地增殖。T细胞增殖在外T区存在B细胞的情况下继续，然后B母细胞经历指数生长并在髓索中分化成浆细胞。在两种反应中，衰老中心都有发展，但MMTV（SW）诱导的衰老中心出现较晚且较小。在MMTV（SW）反应中T区和生发中心激活的大多数T细胞是超抗原特异性的，并且这些细胞在MMTV（SW）注射部位引流的淋巴结中持续数周;这与来自其他次级淋巴组织的超抗原特异性T细胞的选择性损失形成对比。结果表明，这种病毒超抗原，当表达的专业抗原呈递细胞，驱动滤泡外和滤泡B细胞分化，导致病毒特异性抗体的生产。
Mouse mammary tumor virus (MMTV[SW]) encodes a superantigen expressed by infected B cells. It evokes an antibody response specific for viral envelope protein, indicating selective activation of antigen-specific B cells. The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology. T cell priming occurs in both responses, with T cells proliferating in association with interdigitating dendritic cells in the T zone. T cell proliferation continues in the presence of B cells in the outer T zone, and B blasts then undergo exponential growth and differentiation into plasma cells in the medullary cords. Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller. Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection; this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues. The results indicate that this viral superantigen, when expressed by professional antigen-presenting cells, drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.