Host lipids regulate multicellular behavior of a predator of a human pathogen.
Host lipids regulate multicellular behavior of a predator of a human pathogen.
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宿主脂质调节人类病原体捕食者的多细胞行为。
DOI：
10.1101/2024.01.31.578218
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Gerdt,JosephP
中科院分区：
文献类型：
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作者：
Kidner,RiaQ;Goldstone,EleanorB;Laidemitt,MartinaR;Sanchez,MelissaC;Gerdt,Catherine;Brokaw,LorinP;Ros-Rocher,Núria;Morris,Jamie;Davidson,WSean;Gerdt,JosephP
作为动物的共生体，微生物真核生物以多种方式造福和伤害其宿主。模式微真核生物（Capsaspora owczarzaki）是光滑双脐螺（Biomphalaria glabrata）的共生体，可能会阻止寄生虫从蜗牛传播给人类。然而，目前还不清楚是哪些宿主因素决定了Capsaspora的定殖蜗牛的能力。在这里，我们发现，Capsaspora形成多细胞聚集体时，暴露于蜗牛血淋巴。我们确定了聚集的分子线索：血淋巴衍生的磷脂酰胆碱，这在受感染的蜗牛中升高。因此，Capsaspora的聚集可能是其宿主生理状态的反应，并且可能决定其定殖蜗牛和排除寄生性寄生虫的能力。此外，Capsaspora是一种进化模式生物，其聚集可能是动物的祖先。这一发现，一种普遍的脂质诱导Capsaspora多细胞性，表明这种聚集表型可能是古老的。此外，特定的脂质将是进一步聚集研究的有用工具。
As symbionts of animals, microbial eukaryotes benefit and harm their hosts in myriad ways. A model microeukaryote (Capsaspora owczarzaki) is a symbiont of Biomphalaria glabrata snails and may prevent transmission of parasitic schistosomes from snails to humans. However, it is unclear which host factors determine Capsaspora’s ability to colonize snails. Here, we discovered that Capsaspora forms multicellular aggregates when exposed to snail hemolymph. We identified a molecular cue for aggregation: a hemolymph-derived phosphatidylcholine, which becomes elevated in schistosome-infected snails. Therefore, Capsaspora aggregation may be a response to the physiological state of its host, and it may determine its ability to colonize snails and exclude parasitic schistosomes. Furthermore, Capsaspora is an evolutionary model organism whose aggregation may be ancestral to animals. This discovery, that a prevalent lipid induces Capsaspora multicellularity, suggests that this aggregation phenotype may be ancient. Additionally, the specific lipid will be a useful tool for further aggregation studies.