Inorganic/organic hybrid silica nanoparticles as a nitric oxide delivery scaffold
Inorganic/organic hybrid silica nanoparticles as a nitric oxide delivery scaffold
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DOI：
10.1021/cm702526q
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发表时间：
2008-01-08
影响因子：
8.6
通讯作者：
Schoenftsch, Mark H.
中科院分区：
文献类型：
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作者：
Shin, Jae Ho;Schoenftsch, Mark H.
报道了N-二醇二氮烯鎓改性氨基烷氧基硅烷合成后生成的释放一氧化氮（NO）的二氧化硅颗粒的制备和表征。简言之，通过将适量的氨基烷氧基硅烷溶解在乙醇、甲醇和甲醇钠（NaOMe）碱的混合物中来制备氨基烷氧基硅烷溶液。使硅烷溶液与NO（5atm）反应以在氨基烷氧基硅烷上形成N-二醇二氮烯鎓 NO供体部分。然后将四乙氧基或四甲氧基硅烷（TEOS或TMOS）与不同比例的N-二醇二氮烯钥改性的氨基烷氧基硅烷（10-75摩尔%，余量为TEOS或TMOS）混合。最后，在氨催化剂的存在下将硅烷混合物加入到乙醇中以通过溶胶-凝胶过程形成NO供体二氧化硅纳米颗粒。这种合成方法允许制备具有显著改善的NO储存和释放特性的NO递送二氧化硅支架，超过迄今为止报道的关于NO有效载荷的所有大分子NO供体系统（11.26 μ mol中心点mg（-1）），最大NO释放量（357 000 ppb中心点mg（-1））、NO释放半衰期（253 min）和NO释放持续时间（101 h）。通过Si-29核磁共振（NMR）光谱、紫外-可见光谱、化学发光、原子力显微镜（AFM）、气体吸附-脱附等温线和元素分析对N-二醇二氮烯鎓改性的硅烷单体和所得二氧化硅纳米颗粒进行了表征。
The preparation and characterization of nitric oxide (NO)-releasing silica particles formed following the synthesis of N-diazeniumdiolate-modified aminoalkoxysilanes are reported. Briefly, an aminoalkoxysilane solution was prepared by dissolution of an appropriate amount of aminoalkoxysilane in a mixture of ethanol, methanol, and sodium methoxide (NaOMe) base. The silane solution was reacted with NO (5 atm) to form N-diazeniumdiolate NO donor moieties on the aminoalkoxysilanes. Tetraethoxy- or tetramethoxysilane (TEOS or TMOS) was then mixed with different ratios of N-diazeniumdiolate-modified aminoalkoxysilane (10-75 mol %, balance TEOS or TMOS). Finally, the silane mixture was added into ethanol in the presence of an ammonia catalyst to form NO donor silica nanoparticles via a sol-gel process. This synthetic approach allows for the preparation of NO delivery silica scaffolds with remarkably improved NO storage and release properties, surpassing all macromolecular NO donor systems reported to date with respect to NO payload (11.26 mu mol center dot mg(-1)), maximum NO release amount (357 000 ppb center dot mg(-1)), NO release half-life (253 min), and NO release duration (101 h). The N-diazeniumdiolate modified silane monomers and the resulting silica nanoparticles were characterized by Si-29 nuclear magnetic resonance (NMR) spectroscopy, UV-vis spectroscopy, chemiluminescence, atomic force microscopy (AFM), gas adsorption-desorption isotherms, and elemental analysis.